. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 10 D. E. MORSE AND A. N. C. MORSE 100 ,- _ 80 -. None Trps. Papn |3-Gal. c-Gal 0-Glu Hyal Bactri Haiiotis Patella Wu JOOu 200u 200u Sulfatase Figure 5. Effects of purified enzymes used as probes for structural determinants of the cell wall-associated inducer of larval metamorphosis. Trps. = trypsin; Papn. = papain; /J-Gal. = /i-galactosidase; «-Gal. = «- galactosidase; fi-Glu. = /j-glucuronidase; Hyal. = hyaluromdase; sulfatases purified from the bacterium Acrohuctcr acrnt;, the limpet Piilclln vitlgiild. and the abalon
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 10 D. E. MORSE AND A. N. C. MORSE 100 ,- _ 80 -. None Trps. Papn |3-Gal. c-Gal 0-Glu Hyal Bactri Haiiotis Patella Wu JOOu 200u 200u Sulfatase Figure 5. Effects of purified enzymes used as probes for structural determinants of the cell wall-associated inducer of larval metamorphosis. Trps. = trypsin; Papn. = papain; /J-Gal. = /i-galactosidase; «-Gal. = «- galactosidase; fi-Glu. = /j-glucuronidase; Hyal. = hyaluromdase; sulfatases purified from the bacterium Acrohuctcr acrnt;, the limpet Piilclln vitlgiild. and the abalone Haliolis craclierndii. were used in the amounts (enzyme units) indicated. Assays, after treatment and washing of the paniculate samples, were performed in duplicate; all other details as in Figure 4 and Materials and Methods. equivalent amounts of the enzyme-treated and the un- treated morphogen were mixed and assayed together, no reduction in the activity of the untreated morphogen was observed, thus confirming the conclusion that the mol- luscan enzymes inactivated the inducer, and not the larvae. Larvae exposed to molluscan digestive enzyme- treated inducer, and either subsequently (Fig. 4) or si- multaneously (Table II) exposed to untreated inducer, re- sponded normally and completed metamorphosis. The digestive enzyme preparation from Haiiotis that inactivated the coral morphogen (Fig. 4) is a relatively crude mixture containing high quantities of a sulfatase (Spaulding and Morse, 1991) and lower quantities of /3- glucuronidase and several other enzymes. When purified molluscan sulfatase and /i-glucuronidase were tested sep- arately, the sulfatase was a potent inactivator of the cell wall-associated morphogen, whereas jtf-glucuronidase had little if any significant activity (Fig. 5). The data show that sulfatases purified from Haliolis, Patella, and from a bac- terium all inactivate the morphogen, and that the effect of the Haiiotis sulfatase is concentration-dependent
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
