. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 236 G. ROESIJADI 100 60 (XL ! in I t— i o UJ o. 20 O W • o o D CONTROL \iqlt O 7 \ * w v \ V. 10 20 30 TIME (DAYS) FIGURE 1. Percent survival of specimens of Protothaca stanrinca during 30-day exposure to control, 7, 18, 39, or 82 /xg/1 total copper in sea water. an IL 251 atomic absorption spectrophotometer. Bovine serum albumin was used as the protein standard. Deuterium background correction was applied during analyses for metals in column fractions. Calibration standards for the-chromatographic column consisted of b
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 236 G. ROESIJADI 100 60 (XL ! in I t— i o UJ o. 20 O W • o o D CONTROL \iqlt O 7 \ * w v \ V. 10 20 30 TIME (DAYS) FIGURE 1. Percent survival of specimens of Protothaca stanrinca during 30-day exposure to control, 7, 18, 39, or 82 /xg/1 total copper in sea water. an IL 251 atomic absorption spectrophotometer. Bovine serum albumin was used as the protein standard. Deuterium background correction was applied during analyses for metals in column fractions. Calibration standards for the-chromatographic column consisted of blue dextran (void volume), albumin (MW = 67,000 daltons), ovalbumin (43,000), chymo- trypsinogen (25,000) and ribonuclease A (13,000) (Pharmacia Fine Chemicals). All chemicals used in the study were reagent grade or better. Water was deionized using a system of single anion-cation exchange columns and mixed bed anion-cation exchange columns. Glassware was soaked in 50% HNOs followed by 50% HC1 as part of routine cleaning procedures. RESULTS With the exception of the control situation, copper in the various experimental treatments was present mainly as ionic or weakly complexed chemical species. More strongly complexed forms of copper were present at levels of 1 to 3 /tg/1 in tanks with added copper. In the control tank, virtually all the copper was believed to be in the strongly complexed form since ionic/weakly complexed forms were not detectable. The copper concentration for control sea water of jug/1 was consistent with concentrations reported for Sequim Bay sea water (Young, Gurtisen, Apts and Crecelius, 1979) and other natural marine waters (Batley and Gardner, 1978). Copper concentrations as high as those used for exposures (7 to 82 /Ag/1) have been reported for extremely contaminated coastal waters (Wildhauer, Matte and Tucker, 1978). Survival of clams during the 30-day exposure (Fig. 1) was high in controls (= 97%) and slightly reduced at 7 and 18 \ copper
Size: 2361px × 1058px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
