. Agricultural bacteriology; a study of the relation of germ life to the farm, with laboratory experiments for students, microorganisms of soil, fertilizers, sewage, water, dairy products, miscellaneous farm products and of diseases of animals and plants. Bacteriology, Agricultural. 342 LABORATORY WORK No. 18. Fennentation Tubes.—Prepare 200 of bouillon as described in No. 2, adding to it 2 grams lactose and adjust the reaction to the neutral point. To a second lot of bouillon add the same amount of dextrose and to a third lot the same amount of saccharose. After dissolving and filtering
. Agricultural bacteriology; a study of the relation of germ life to the farm, with laboratory experiments for students, microorganisms of soil, fertilizers, sewage, water, dairy products, miscellaneous farm products and of diseases of animals and plants. Bacteriology, Agricultural. 342 LABORATORY WORK No. 18. Fennentation Tubes.—Prepare 200 of bouillon as described in No. 2, adding to it 2 grams lactose and adjust the reaction to the neutral point. To a second lot of bouillon add the same amount of dextrose and to a third lot the same amount of saccharose. After dissolving and filtering pour into fermentation tubes, enough to fill the closed arm and half the bulb (Fig. 63). A dozen or more tubes of each of these bouillons should be prepared and labeled. Sterilize by steam- ing on three days, or by not over 15 minutes in the autoclave. If gas collects in the closed arm remove by tilting the tube. No. 19. Testing Characters of Bacteria.— Several isolated and purified cultures of bacteria have been prepared in No. 7. After having prepared the several culture media above de- scribed, use them as follows: Make a fresh agar slant from each purified bacterial culture and allow to grow about 24 hours. If possible use a culture of B. coli for one of the series of tests. Then inoculate with a small quantity of the growth the following: (a) Two agar slants. (5) One gelatin stab. This is made by dipping a straight platinum needle into the bacteria and then thrusting it straight into the gelatin of a gelatin tube, in the middle of the tube, and forcing the needle to the bottom, carefully withdrawing without disturbing the gelatin, (c) A fermentation tube of each of the three sugars, (d) Two milk tubes, (fi) Two litmus milk tubes, (f) Two po- tato tubes, inoculating the potato on its surface only. Place one of the agar tubes, one of the milk tubes, litmus milk tubes, and potato tubes in the incubating oven at gS°. Place all others at room temperature. Allow to grow sev
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteriologyagricult
