. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 456 MANGUM ET AL. Measurements on blood and urine samples pH was measured with a liquid junction capillary electrode (Radiometer Corp.). Ammonia concentration was determined by the phenol hypochlorite method (Solorzano, 1969), using ml samples. At high concentrations, we found it necessary to dilute the samples with distilled water and to allow two hours for completion of the reaction. In these cases, the background concentration of the ammonia in H2O was subtracted from the results. Otherwise, the procedure was unchange
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 456 MANGUM ET AL. Measurements on blood and urine samples pH was measured with a liquid junction capillary electrode (Radiometer Corp.). Ammonia concentration was determined by the phenol hypochlorite method (Solorzano, 1969), using ml samples. At high concentrations, we found it necessary to dilute the samples with distilled water and to allow two hours for completion of the reaction. In these cases, the background concentration of the ammonia in H2O was subtracted from the results. Otherwise, the procedure was unchanged. Sodium concentration was measured with an EEL flame photometer (Oglesby, 1970), and chloride with a Buchler-Cotlove chloridometer. Osmolality was de- termined with an Osmette freezing point osmometer, and total solids with an Atago refractometer. The absorbance of blood was measured at 340 nm (Beckman DK-2 spectro- photometer) after dilution of the sample with distilled water (1:39). The con- centration of hemocyanin was computed from the molecular extinction coefficient given by Nicker son and van Holde (1971). Oxygen carrying capacity at 159 torr was calculated from these results on the assumption that of the total weight of the hemocyanin molecule is copper, and that Cu combines with molecular oxygen in the ratio 2: 1 (van Holde and van Bruggen, 1971). A test of this procedure, made by comparing the results with those of more time-consuming but direct mea- surements (Mangum, Freadman and Johansen, 1975), indicates that it is equally accurate. 200r - o E O) 100 80 O •> 40. o 1 Body weight [kg] FIGURE 1. The rate of oxygen consumption (VO2) in Limidus polyphcmus acclimated to osed circles) and 8 (open circles) %e salinity and 22° C. The 95% confidence interval ; the regression line describing the combined data. The slope of that line is — (± ) and the correlation coefficient (r) is Please note that these images are extracted from
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
