. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 416 P. V. DUNLAP. Figure 2. SEM of ponyfish {Gazza minuta) light organ tubules, which house the bacterial symbionts {Photobacterium leiognathi). Light organ saccules, apparently destroyed in preparation of the light organ for SEM, are not visible. Note the scarcity of capillaries (as noted by Bassot, 1975) and the density and uniformity of shape of the bacterial cells in the tubules. Size bar = 25 ^m. cases were non-motile. Using the Leifson method (Doetsch, 1981), staining for flagella gave negative results, whereas control
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 416 P. V. DUNLAP. Figure 2. SEM of ponyfish {Gazza minuta) light organ tubules, which house the bacterial symbionts {Photobacterium leiognathi). Light organ saccules, apparently destroyed in preparation of the light organ for SEM, are not visible. Note the scarcity of capillaries (as noted by Bassot, 1975) and the density and uniformity of shape of the bacterial cells in the tubules. Size bar = 25 ^m. cases were non-motile. Using the Leifson method (Doetsch, 1981), staining for flagella gave negative results, whereas controls grown overnight in SWC broth and on SWC agar showed active motility and polar flagellation (as described for P. leiognathi in culture; Reichelt and Baumann, 1973). Motility of bacteria in light organ homogenates developed after 6 to 12 hours at 24°C. Four to five percent of the 1200 bacteria examined from one fish {G. minuta) were visibly in division. Using a regression formula that relates frequency of dividing bacterial cells (FDC) to growth rate of a natural population, \n ix = FDC — (Newell and Christian, 1981), the growth rate of the bacteria was estimated to be h"'. In culture, growth rates of h~' or higher are common for P. leiognathi. Thus, the light organ bacteria in situ double slightly less often than once. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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