. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 126 (,. \Y. KIDDER AND V. C. DEWEY The addition of thiainine to either the heated casein or filtered heated casein plus dethiaminized alfalfa extract has very little effect. The cultures maintain a slightly higher level at 11 days duration but the shape of the growth curves are almost identical. "When a per cent solution of a biuret negative casein hydrolysate plus per cent 1-tryptophane was used as a basic medium it was found that growth was impossible beyond the first transplant, even when thiamine wras add
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 126 (,. \Y. KIDDER AND V. C. DEWEY The addition of thiainine to either the heated casein or filtered heated casein plus dethiaminized alfalfa extract has very little effect. The cultures maintain a slightly higher level at 11 days duration but the shape of the growth curves are almost identical. "When a per cent solution of a biuret negative casein hydrolysate plus per cent 1-tryptophane was used as a basic medium it was found that growth was impossible beyond the first transplant, even when thiamine wras added. Inasmuch as unheated casein and filtered heated casein give slow but indefinitely transplantable growth the acid hydrolysis must have destroyed some factor or factors, other than thiamine, necessary for growth. Excellent growth resulted, however, when de- TABLE III Medium Generation time in hours Population per ml. at end of log. phase Maximum yield cells/ml. Population per ml. at 11 days Size of cells at 1 1 days (av. 20 measurements) Gelatin 2 per cent 2,600 12,000 2,100 22M X Gelatin 2 per cent + B! 2,400 67,000 31,000 91. SM X 34M Gelatin 2 per cent + A 18,000 140,500 47,000 51ju X 24M Gelatin 2 per cent + A + B! 17,200 161,000 52,000 X 22M A = heat and alkali treated alfalfa extract; BI = thiamine 1 microgram/ml. All media contains salts, riboflavin ( microgram/ml.) and 1-tryptophane ( per cent).. 48 72 96 120 144 HOURS 168 192 216 240 264 FIGURE 3. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biologica
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