Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . ilaria, and trypanosomes. It affords evidence of increasedor decreased rouleaux formation, number, deformities, and degenerations,as well as of the amount of hemoglobin of the red cells; the presence of aleucocytosis or of a leucopenia and of ameboid movement of the , care must be taken to avoid premature conclusions from such studyand to institute further examinations of the stained specimen to clear up doubt-ful points. The observer must be on his guard lest he mist
Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . ilaria, and trypanosomes. It affords evidence of increasedor decreased rouleaux formation, number, deformities, and degenerations,as well as of the amount of hemoglobin of the red cells; the presence of aleucocytosis or of a leucopenia and of ameboid movement of the , care must be taken to avoid premature conclusions from such studyand to institute further examinations of the stained specimen to clear up doubt-ful points. The observer must be on his guard lest he mistake the normalBrownian movement in the protoplasm of the cells for ameboid or parasiticmovement. Curious phenomena are observed in the fresh specimen as theblood dries and should not be misinterpreted. The various characteristicsof fresh blood will be taken up in detail later. The introduction of the ultra-condenser or dark-field illuminator has opened up a field of great possibilitiesin the examination of specimens of fresh blood, especially when malarialparasites or spirochaetas pallidae are Fig. 138.—Preparation of smears with two glass slides. {Da Costa.) (2). Preparation of Smears. To prepare blood smears, which are to be later examined in the stainedcondition, one may spread the blood in capillary layers on slides or betweencover-glasses. The former method is the one used in the writers laboratoryand has given excellent and satisfactory results. A fair-sized drop of blood iscollected on one end of a clean dry slide, held between the thumb and secondand third finger of the left hand. A second slide is held in the same mannerby the right hand, but at an angle of 45 degrees to the first one and touchingthe drop of blood. Allow the blood to spread out by capillarity along theedge of the second slide. As soon as this occurs, draw the drop of bloodalong the first slide with a clean sweep, exerting little pressure with the secondslide and maintaining the angle of 4
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