. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 430-445m;j — logIo/I = T" 10 20 Seconds 30 40 Fig. and 8. The kinetics of photodissociation recombination of the cytochrome homogenates, bakers' yeast cells, A. pasteurianmn, and Bacillus suhtilis^^, and find £5^^^, = 12 cm-^ X mil/"i fQj- lY^Q cytochromes of type a^. (Reference 14 gives detailed data on the experimental controls and also the method of calculating the results.) In our previous studies the sensitivity of the appara
. Cell chemistry; a collection of papers dedicated to Otto Warburg on the occasion of his 70th birthday. Warburg, Otto Heinrich, 1883-; Biochemistry. 430-445m;j — logIo/I = T" 10 20 Seconds 30 40 Fig. and 8. The kinetics of photodissociation recombination of the cytochrome homogenates, bakers' yeast cells, A. pasteurianmn, and Bacillus suhtilis^^, and find £5^^^, = 12 cm-^ X mil/"i fQj- lY^Q cytochromes of type a^. (Reference 14 gives detailed data on the experimental controls and also the method of calculating the results.) In our previous studies the sensitivity of the apparatus was insufficient to give any quan- titative idea of the molecular extinction coef- ficients of the "CO-binding pigment" of S. albus. We now have increased the sensitivity, and satis- factory kinetic data may be obtained as in Fig. 8. A preliminary value can be given for the molecu- lar extinction coefficient of the band at 546 m/u, £ = 5 cm-i X milf-i, about half that of the value for myoglobm-CO at 580 m/x ( cm"^ X mil/~^)^2. The extinction coefficient at 589 m^u is very small compared to that of the yeast enzyme and this emphasizes the difference be- tween this CO-binding pigment and cytochrome The photochemical action spectrum One of us (L. C.) has recently developed an appa- ratus for measuring photochemical action spectra in a drop of bacterial suspension by means of the platinum microelectrode^^. This method utilizes the steady-state system developed by Connelly and Brink^^ in their studies of the respiration of nerve. A steady-state in oxygen tension results from the balance between inward diffusion of oxygen and utilization of oxygen by the nerve tissue. The same fitg-CO compound in bakers' yeast cells as measured by the method of Fig. 4 (Expt. measured by the method of Fig. 4. Il- luminating light is 589 m/^ (Expt. 144).. Fig. g. The kinetics of photodissocia- tion and recombination of "CO-binding pigment" in a suspension of S/
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