. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SYMBIONTS OF LASIODERMA AND STEGOBIUM 421 following vitamins of the B-complex were added (expressed as jug. per gram of the dry diet) : thiamin 25, riboflavin , nicotinic acid 25, pyridoxin , pantothenic acid 25, choline chloride 500, inositol 250, folic acid and biotin In some cases the water-insoluble residue of yeast was used in the place of biotin (Fraenkel and Blewett, 1943). The tests were carried out in 2 X 1 inch shell vials closed by one-holed corks with muslin tops. Ten newly hatched larvae were ad
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SYMBIONTS OF LASIODERMA AND STEGOBIUM 421 following vitamins of the B-complex were added (expressed as jug. per gram of the dry diet) : thiamin 25, riboflavin , nicotinic acid 25, pyridoxin , pantothenic acid 25, choline chloride 500, inositol 250, folic acid and biotin In some cases the water-insoluble residue of yeast was used in the place of biotin (Fraenkel and Blewett, 1943). The tests were carried out in 2 X 1 inch shell vials closed by one-holed corks with muslin tops. Ten newly hatched larvae were added to each tube and each test was performed in duplicate. Each tube contained two grams of food. All tests were carried out in a constant-temperature-humidity chamber of 27° C. and 70% relative humidity. MYCETOME. FIGURE 1. Intestine of the adult Lasiodcnua sen-iconic, showing the mycetoms at the junction of fore- and midgut. For the study of the symbionts in situ, sections were cut and stained with Dela- field's hematoxylin and eosin or orange G. Smears of mycetomic tissue were stained with Wright's stain or were smeared on to the slide with a drop of India ink. The latter method proved very suitable for the study of the shape of the cells. For the graphic representation of results, the total number of adults formed was plotted against time. Both Lasiodcnna and Stcyolnum spin a cocoon previous to pupation. Most of these cocoons are fixed on to the glass wall of the vials leaving open a window to the inside of the cocoon through which the insects can be observed. Thus the time of pupation and emergence of the adults can be accurately determined by frequent Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hol
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
