. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 176 EDWIN P. MARKS. Theoretical •96 Practical 1 5 20 25 30 35 Age in vivo (Days) 40 45 50 55 FIGURE 8. Effect of age in vivo on the induction by /3-ecdysone (25 /*gd) of cuticle deposi- tion and seta formation in cockroach leg regenerates; regression for cuticle deposition (O) is y = + ; r = and regression for seta formation (X) from 15 to 30 days y = ; r = is we found that 75% of 30-day-old regenerates that were induced to molt also hore setae; that 54% of 35-day-old regenerates developed
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 176 EDWIN P. MARKS. Theoretical •96 Practical 1 5 20 25 30 35 Age in vivo (Days) 40 45 50 55 FIGURE 8. Effect of age in vivo on the induction by /3-ecdysone (25 /*gd) of cuticle deposi- tion and seta formation in cockroach leg regenerates; regression for cuticle deposition (O) is y = + ; r = and regression for seta formation (X) from 15 to 30 days y = ; r = is we found that 75% of 30-day-old regenerates that were induced to molt also hore setae; that 54% of 35-day-old regenerates developed without exogenous hormone, and that by 45 days, the new cuticle with setae was present at the time of explanta- tion (Marks and Leopold, 1971). The average stadium was 52 days with a range of 45-60. Thus the slopes for cuticle deposition and seta formation met at an TABLE II The effect of puromycin on the induction by 0-ecdysone of cuticle deposition and seta formation in vitro Dose (fig/ml) Days in vitro Number specimens Percentage response 0-ecdysone Puromycin Cuticle Seta None None 3 15 0 0 None 3 15 96 33 3 15 93 0. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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