. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. at the boiling point filter the agar and dis-tribute into culture tubes. 9. The tubed agar should be sterilized in the auto-clave for 5 minutes. For agar slants each tube should contain about 7 be filled one-third of the length of the tube. Forplating, the tube should be half filled and contain Slants are prepared by allowing the agar aftersterihzation to cool in a slanting position. If it is de-sired to slant a large number a whole basket may beput in a slanting position. When a few tubes
. A laboratory guide in bacteriology, for the use of students, teachers, and practitioners. at the boiling point filter the agar and dis-tribute into culture tubes. 9. The tubed agar should be sterilized in the auto-clave for 5 minutes. For agar slants each tube should contain about 7 be filled one-third of the length of the tube. Forplating, the tube should be half filled and contain Slants are prepared by allowing the agar aftersterihzation to cool in a slanting position. If it is de-sired to slant a large number a whole basket may beput in a slanting position. When a few tubes only 22 LABORATORY GUIDE IN BACTERIOLOGY are required they may rest with the plugged end on aglass rod or rubber hose until the agar has solidified. For the purpose of filtering media heavy filterpaper (Schleicher and SchuU No. 598) of the bestquality only should be used. This is especially im-portant when filtering agar or gelatin. After the filter Q Fig. 12 Apparatus for Filtering Media 0. Filter b. Large funnel c. Small funnel d. Rubber hose e. Pinchcocic /. Pipette g. Culture tube. has been folded and inserted into the funnel hot watershould be run through the filter, until it is soakedand warm. Method of folding filters (Fig. 13).—a and b) Takea square piece of filter paper twice as wide as the depthof the funnel and fold to half the size so as to makeNo. I cover No. 2. (Compare with Fig. 13.) BACTERIOLOGICAL TECHNIC 23 c) Fold this to make i cover 2 and 3 cover 4 (resultFig. 13c). It consists of four layers and forms a square. d) Fold the upper part, consisting of two layers,from I to 2 (Fig. 13^). The shaded triangle, 2-3-4,now has six layers; the other, 1-3-4, two layers.
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