. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 420 F. TABLIN AND J. LEVIN. h'igurc 2 \. Name anu-boiste h\ed bv tin; method ol Hovles a •) are seen among the cortical actin lilaments. • (Pro\ided h> Paula [;. Stenherg. unpuh. obs.) This distinctive arrangement was observed in virtually all activated cells. Neither microtubules nor distinct ar- rays ofthin filaments appeared to be associated with sites ofexocytosis. Endotoxin-activated amebocytes Amebocytes exposed to bacterial endotoxm appeared hi he rapid!} activated and underwent extensive shape change and
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 420 F. TABLIN AND J. LEVIN. h'igurc 2 \. Name anu-boiste h\ed bv tin; method ol Hovles a •) are seen among the cortical actin lilaments. • (Pro\ided h> Paula [;. Stenherg. unpuh. obs.) This distinctive arrangement was observed in virtually all activated cells. Neither microtubules nor distinct ar- rays ofthin filaments appeared to be associated with sites ofexocytosis. Endotoxin-activated amebocytes Amebocytes exposed to bacterial endotoxm appeared hi he rapid!} activated and underwent extensive shape change and degranulation. Subsequent!}, most cells con- tained on!} a small residual population of granules which were located adjacent to the nucleus. In manv cells, there was a coalescence of granules and granule membrane fu- sion was apparent. Unlike ainebocUes activated hv con- tact with an mdotoxin-frec surface (and in which cx- ocytosis occurred following movement of granules to the plasma in nibranc [Fig. 5]). cndotoxin-lrealcd amebocytes u\ . I to secrete their granules from a more central h- • iihm the cell (l-ig. (>). 1 ndotoxin-actival bocyteswere more spherical in shape, with numeioir. hlopodia. I me lilopodia con- tained bundles of actin lilamenls. but the overall arrange- ment ofthin filaments was vastly different from either native or surface-activated amebocytes (Fig. 6). Short pieces of thin filaments remained associated with the plasma membrane, and occasional!}, extended into the cytoplasm as isolated groups of two or three filaments, without apparent organi/ation. A large area of cytoplasm between the plasma membrane and the now centrally lo- cated granules and nucleus contained sparse, random!} arranged thin lilamenls. At the outermost aspect of the centralized granules, there was a loosely organi/ed bun- dle of actin filaments whose ana} was similar to the cor- tical actin seen in native cells (1 tg. 6). I he MB surrounded the centrali/ed granules and was l
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
