. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. TRYPSIN SENSITIVITY AT FERTILIZATION 487 The data of Figure 1 clearly show that treatment with trypsin in the appropriate concentration selectively inhibits pair formation. This effect is assumed to result from the proteolytic action of the enzyme since the effect is not obtained in the presence of trypsin-inhibitor. The failure to pair could be due to an enzymatic interference with the process of pair formation or to the enzymatic elimination of functional components at the prospective attachment sites. Such functional comp
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. TRYPSIN SENSITIVITY AT FERTILIZATION 487 The data of Figure 1 clearly show that treatment with trypsin in the appropriate concentration selectively inhibits pair formation. This effect is assumed to result from the proteolytic action of the enzyme since the effect is not obtained in the presence of trypsin-inhibitor. The failure to pair could be due to an enzymatic interference with the process of pair formation or to the enzymatic elimination of functional components at the prospective attachment sites. Such functional components essential for papillar attachment and adhesion could exist either before the mating type reaction, or could be formed or set into action only after the mating type reaction has ensued. 90 80 70. 50 40 30 20 10 5 10 15 20 25 30 35 40 45 50 55 time after mixing of the sexes FIGURE 3. Trypsin-sensitive phase of pair formation in C. monvusii. After mixing of both gamete types samples were taken at 5 min intervals, incubated with trypsin for 15 min and fixed afterwards (A'-data). Compared with the normal formation rate of pairs (Curve A) each A' value has to be related to the percentage of pairs originally present in the sample in question as well as to the percentage to which the pairing has meanwhile proceeded. According to Figure 1 (curves A and C) trypsin prevents any pairing when added simultaneously with the mixing of the two gamete types. About 5-7 min elapse between the initial agglutination and the appearance of free-swimming and lixation-resistent pairs. If pairing is prevented by the enzymatic elimination of preexisting attachment sites, the 5-7 minute time lapse would have to be sufficient to eliminate the sites. However, gametes which were pretreated with trypsin for twice that length of time, did not show any inhibition of the pairing if sufficient trypsin-inhibitor was added at the time of mixing of the sexes (Fig. 2). Evidently, inhibition of the pairin
Size: 1310px × 1908px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
