. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. THE PLATE PEOCESS 55 During tliese operations some plates should be under- going sterilisation in the hot-air steriliser at a temperature of 130° C. A box of sheet iron may be used with advantage for this purpose, and is, moreover, capable of containing a larger number of plates (fig. 24). After cooling, three plates, which must only be seized by their corners, are next taken out of the box and laid one after the other on Koch's plate-making apparatus, on which they are cooled
. Manual of bacteriology for practitioners and students, with especial references to practical methods. Bacteriology. THE PLATE PEOCESS 55 During tliese operations some plates should be under- going sterilisation in the hot-air steriliser at a temperature of 130° C. A box of sheet iron may be used with advantage for this purpose, and is, moreover, capable of containing a larger number of plates (fig. 24). After cooling, three plates, which must only be seized by their corners, are next taken out of the box and laid one after the other on Koch's plate-making apparatus, on which they are cooled under a bell-glass. In the absence of a hot-air steriliser the plates may be sterilised in the interior of an oven, or in the gas or spirit flame by holding them by the corners in the fingers and heating both sides over the Fi3. 24.—Case pp Sheet-iron for holding the PLAiTiS. The plate apparatus (see p. 27) consists of a triangle with feet formed by levelling screws, on which rests a glass vessel covered with a thick plate of glass and filled before use with iced water. It is rendered horizontal with the aid of a spirit-level and covered with a bell-glass. The sterilised plates having cooled under this bell, the first of the inocu- lated test-tubes is then unplugged, its upper edge is heated in order to sterilise that part over which the gelatine has to flow, and its contents are poured from a small height upon the plate in such a manner that the gelatine spreads out over it in a fairly thin layer. In a short time the gelatine sets under the bell-glass, and the plate is then brought into a moist chamber and laid upon either little glass benches. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Schenk, Samuel Leopold, 1840-1902. London, Longmans, Green
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Keywords: ., bookcentury1800, bookdecade1890, booksubjectbacteri, bookyear1893
