. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. GLUTATHIONE AND ANAEROBIOSIS 335. FIGURE 2. Polarograms of the same hind-gut sample from Cryptocercus: (A) untreated, (B) following air passage for one hour, and (C) following nitrogen gas flow, 20 minutes (sensitivity X 5; pH approximately at start). Patterson, Lazarow and Levey (1949) report alloxan in excess reacts with the sulhydryl of GSH forming an unknown substance with an absorption spectrum maximum at 305 m/x. By this method 3 X 10~4 M GSH has been identified in hind-gut content cleared of cellulose and pro
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. GLUTATHIONE AND ANAEROBIOSIS 335. FIGURE 2. Polarograms of the same hind-gut sample from Cryptocercus: (A) untreated, (B) following air passage for one hour, and (C) following nitrogen gas flow, 20 minutes (sensitivity X 5; pH approximately at start). Patterson, Lazarow and Levey (1949) report alloxan in excess reacts with the sulhydryl of GSH forming an unknown substance with an absorption spectrum maximum at 305 m/x. By this method 3 X 10~4 M GSH has been identified in hind-gut content cleared of cellulose and protozoa. The authors mentioned also report equivalent molecular amounts of cysteine, cysteinylglycine, glutamylcysteine, and ascorbic acid in the presence of GSH lower the value of GSH determination. C. Bioassay Loomis (1955a, 1955b) showed that a concentration of 1C)-5 M GSH elicits a characteristic feeding response in starved Hydra littoralis. A 1 :3000 dilution of Cryptocercus gut content induced a strong response, indicating a concentration of GSH somewhere in the order of 10~3 M. This bioassay, however, is no longer specific for GSH. Lenhoff and Bovaird (1960) obtained the same response with trypsin, and Cliffe and Waley (1958) obtained it with ophthalmic acid. 3. The apparent source of GSH Since variables involved with polarograph operation were held constant, slight variations in GSH concentration, observed in fresh unaltered samples, must be inherent in sample fluids. The ratio between number of cells and surrounding fluid volume probably varies between hosts. If GSH is protozoan and/or bacterial in. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
