. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LIML/LUS AMEBOCYTE CYTOSKELETON 59. Figure 2. Microtubule distribution in unactivated cells; phase contrast and fluorescence microscopy, (a-c) A field of suspension-labeled cells, observed with phase contrast, anti-tubulin, and DAPI labeling, respec- tively. The marginal band (MB) in all cells follows ellipsoidal cell contour (a b), with nuclei in the interior (c). (d, e) Two cells viewed on edge; phase contrast and anti-tubulin labeling, respectively. Flattened morphol- ogy is evident, with MB at the planar extremities
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LIML/LUS AMEBOCYTE CYTOSKELETON 59. Figure 2. Microtubule distribution in unactivated cells; phase contrast and fluorescence microscopy, (a-c) A field of suspension-labeled cells, observed with phase contrast, anti-tubulin, and DAPI labeling, respec- tively. The marginal band (MB) in all cells follows ellipsoidal cell contour (a b), with nuclei in the interior (c). (d, e) Two cells viewed on edge; phase contrast and anti-tubulin labeling, respectively. Flattened morphol- ogy is evident, with MB at the planar extremities (e; arrowheads), (f) Higher magnification view of MB microtubules in another cell. The MB consists of one major circumferential microtubule bundle, with few micro- tubules evident elsewhere. arrows), with adjacent hazily labeled areas that are attrib- utable to cortical F-actin being observed in face view. Additional structural features of unactivated cells: TEM observations To visualize cytoskeletal elements and cellular organelles in greater detail, fixed, unactivated cells were examined in TEM thin sections (Fig. 5). In addition to the electron-dense granules found throughout the cell, major components vis- ible in survey views included nucleus, mitochondria, and Golgi apparatus; there was no surface-associated canalicular membrane system such as is found in non-mammalian ver- tebrate thrombocytes and mammalian platelets (Fig. 5a). Examination of numerous mature granules in TEM thin sections showed that those closest to the cell surface were always separated from the plasma membrane by a cytoplas- mic gap —50 nm thick (Fig. 5b. c). At higher magnifica- tions, MB microtubules and centrioles (Fig. 5d-g) were visible, with the MB routinely observed at the cell periphery in various sectional views ( Fig. 5d e). In cross. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appeara
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