. Carnegie Institution of Washington publication. . Fig. 60.' This organism grows slowly on gelatin without liquefaction. In a stab-eulture in nutrient gelatin No. 478 at the end of 41 days, at 170 to 220 C, there was a thin line of growth along the needle-track, best toward the top, and a dense, rather dry, slightly rough- ened, bright, buff-yellow surface growth 7 mm. in diameter. Fig. 61 a shows its appearance in gelatin stabs. In +10 nutrient gelatin, in rather thin sowings in Petri-dish poured-plates, at the end of 7 days, at io° to 200 C, the surface colonies of Bacterium stewarti under
. Carnegie Institution of Washington publication. . Fig. 60.' This organism grows slowly on gelatin without liquefaction. In a stab-eulture in nutrient gelatin No. 478 at the end of 41 days, at 170 to 220 C, there was a thin line of growth along the needle-track, best toward the top, and a dense, rather dry, slightly rough- ened, bright, buff-yellow surface growth 7 mm. in diameter. Fig. 61 a shows its appearance in gelatin stabs. In +10 nutrient gelatin, in rather thin sowings in Petri-dish poured-plates, at the end of 7 days, at io° to 200 C, the surface colonies of Bacterium stewarti under the Zeiss 16 mm. objective and 12 comp. ocular were small, not perfectly circular, not uniformly granular, more or less finely fissured, the margins not entire and sometimes even more or less decid- edly notched; the buried colonies were globose-lobulated and less than 1 mm. in diameter. This may not be a con- stant phenomenon. In streaks on Loefner's solidified blood-serum Bad. stewarti made a good bright buff-yellow growth, but without liquefaction. The cultures were under observation for 32 days. On agar plates it grows slowly, forming small round yellow colonies, the buried ones being very small. In agar streak-cultures it gives a smooth trans- lucent yellow slime which is usually paler than that of Bad. hyaciiithi (fig. 62), and frequently lobed on the margins. Old streak-cultures are usually thin and somewhat dry, and the older bacterial layer generally bsars numerous small raised, wet-shining, yellow colo- nies on its surface. Jagged X-shaped crystals and short prismatic crystals are not uncommon (fig. 63). In agar stab-cultures at the end of 16 days there was a moderate growth the whole length of the needle- track and a distinct surface growth some millimeters ' in diameter. Even where there were several stabs in one tube the growths did not cover the entire surface of the agar or coalesce; the color was between saffron- yellow and deep chrome (Ridgway). At the end of
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Keywords: ., bookauthorcarnegie, bookcentury1900, bookdecade1910, bookyear1914
