. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 328 L. S. QUACKENBUSH AND L. L. KEI I I N. Ic I vipn^-. • ablati Sample Proteins (mg) Hepatopancreas llemolymph ("rude I Pellet Supernatanl Sample ' ii 04 ( ± ± ± ± DPM/MG/Hour ± ( ± ( ± Ovarj Hepatopancreas Hemolvmph Crude Extract Pellet Supernatant ± 865 - 181 ± 8,536 ± ± 755 ± 567 ± 123 792 ±71 778 ± 79 Ml values are means ± one standard deviation, n = 12 for all cases, 2 replicates. C'rude extract is the
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 328 L. S. QUACKENBUSH AND L. L. KEI I I N. Ic I vipn^-. • ablati Sample Proteins (mg) Hepatopancreas llemolymph ("rude I Pellet Supernatanl Sample ' ii 04 ( ± ± ± ± DPM/MG/Hour ± ( ± ( ± Ovarj Hepatopancreas Hemolvmph Crude Extract Pellet Supernatant ± 865 - 181 ± 8,536 ± ± 755 ± 567 ± 123 792 ±71 778 ± 79 Ml values are means ± one standard deviation, n = 12 for all cases, 2 replicates. C'rude extract is the initial tissue homogenate, Pellet is the protein precipitated bv the antihod> to vitellogenin, Supernatant is the protein not precipitated bv the antibod>. Recovers of both label and protein was between SO-901; tor these assays. lion from the crude material (traction #29. Rf = ). Fraction -2^ inhibited Vg 14C leucine incorporation. It was specific to Vg proteins. Fraction #29 had no signifi- cant effect on I4C leucine incorporation into proteins other than Vg. represented by the supernatant in the im- munoassay (Table II). Both the crude starting material and material from the column void volume inhibited MC leucine incorporation into both Vg and non-Vg proteins, demonstrating non-specific protein synthesis inhibition (Table II. Fig. 6). Based on the si/e estimate for fraction #29 ( ± 500 daltons) the material in this fraction was biologically active at < 10 ".I/protein. Discussion The primary source of crustacean egg yolk proteins was first suggested to be extra-ovarian (Wallace cl V Ganion and Kessel, 1972; Wolin cl til. 1973: Schade and Si ivers, 1980). Direct demonstration of pioiem synthi o\anan tissue supported the new view that the • ontribution to overall egg yolk protein svnth mficant. I he relalivclv slow rale M| ovarian prolcm s in isolated ovarian tissue supported the argument >'iat extra-ovarian tissue also contrib
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
