. Bacteria in relation to plant diseases. Bacteria; Plant diseases. SCHUSTER S GERMAN POTATO ROT. 275 exposed to normal conditions in a hothouse it is not parasitic. I could not get any infec- tions either by rubbing it into stomata or by needle-puncture into leaves or stems. The organism grows in milk about as described, but on agar-streaks ( + 15) I did not obtain the cross-folds and am inclined to regard this as an accidental phenomenon due to dry agar or to some other peculiarity of medium. The organism was tried on various sugars and alcohols in the presence of river-water and Witte's pep
. Bacteria in relation to plant diseases. Bacteria; Plant diseases. SCHUSTER S GERMAN POTATO ROT. 275 exposed to normal conditions in a hothouse it is not parasitic. I could not get any infec- tions either by rubbing it into stomata or by needle-puncture into leaves or stems. The organism grows in milk about as described, but on agar-streaks ( + 15) I did not obtain the cross-folds and am inclined to regard this as an accidental phenomenon due to dry agar or to some other peculiarity of medium. The organism was tried on various sugars and alcohols in the presence of river-water and Witte's peptone (1 per cent). Contrary to Schuster, it ferments grape-sugar and galactose with production of an acid, but a similar solution with cane-sugar gave an alkaline reaction (fermentation tubes, 11 days). The same results were obtained in two repe- titions (twenty-eighth day and later). The other car- bon foods tested in fermen- tation tubes were fructose, lactose, maltose, mannit, and glycerin. All of these gave an alkaline reaction (eleventh day). In two repetitions the same result was obtained after longer periods. There was no gas formation and no clouding in the closed end. As stated, the organism did not rot potato tubers at 37° C, nor could we get any evi- dence of growth above 34° C. in + 15 peptone-bouillon or on steamed potato. It grew better at 300 C. than at 340 C. but less well than at26°C. It grows at i° C. Its thermal death-point is 510 C, approximately. It grows in bouillon over chlo- roform without retarda- tion. It grows in +15 peptone-bouillon with 4 per cent sodium chloride and slowly with 5 per cent, but not with 6 per cent. It does not grow in Cohn's solution. It grew in pep- tone bouillon from —30 to +25. It grew about as well in —15 and —30 as in o or +25. It grew well in Uschinsky's solution, forming a copious pellicle and after a time a pale green It is sensitive to dry air (dead on cover slips at end of 4 days). It liquefied gel
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