. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 01 345678. 012345678 012345678 Day of Culture Figure 2. Growth and development of in ovo control embryos, transplanted embryos, and ex ovo ( capsular fluid. pH ) cultured embryos at 23 °C. The growth (length of embryos) of stage E15 (A) and E20 (B) embryos cultured v was significantly slower than that of control embryos (Student's t test. P < at tune points marked by asterisks, n = 1 for each of the c.\ nvn culture trials [10-15 embryos in each trial], n = 6 and 5 for control stage E15 and E20 embryos,
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 01 345678. 012345678 012345678 Day of Culture Figure 2. Growth and development of in ovo control embryos, transplanted embryos, and ex ovo ( capsular fluid. pH ) cultured embryos at 23 °C. The growth (length of embryos) of stage E15 (A) and E20 (B) embryos cultured v was significantly slower than that of control embryos (Student's t test. P < at tune points marked by asterisks, n = 1 for each of the c.\ nvn culture trials [10-15 embryos in each trial], n = 6 and 5 for control stage E15 and E20 embryos, respectively I. In contrast, growth of transplanted stage ELS and E20 embryos was very similar to their control counterparts. Slight differences were seen only at later stages of culture in transplanted E15 embryos (marked by asterisks; / test. P n = 6). The development of the same stage E15 (C) and E20 (D) embryos cultured ex ovo was much slower than that of control embryos (asterisks: / test. P < ). In transplanted stage E15 and E20 embryos, however, the development was very similar to their control counterparts. Small differences were only seen at later stages of culture in transplanted E15 embryos (asterisks; t test. P ). depleted as embryonic development proceeds (Morrill, 1982; Stockmann-Bosbach and Althoff, 1989). In most cases, the incision in the capsule membrane re-sealed within 12 h after transplantation. In striking contrast to the ex ovo trials, the growth and development of transplanted embryos was very similar to that displayed by control embryos within intact egg capsules (Fig. 2). At 23 °C, the growth and development of trans- planted stage El5 embryos were identical to the controls in the first 3-4 days but slightly slower thereafter (Fig. 2A, C). When transplanted at stage E20 or older, however, the lengths (Fig. 2B) and embryonic stages (Fig. 2D) reached by transplanted embryos at various days were identical to controls (Student's t test,
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
