. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 226 Experimentation upon Animals When, in studying Pfeifier's phenomenon and similar conditions, it is desirable occasionally to withdraw drops of fluid from the ab- dominal cavity, a small opening can be burned through with a blunt needle. This does not heal readily, and through it, from time to time, a capillary pipet can be introduced and the fluids withdrawn. Small animals, such as rabbits and guinea-pigs, can be held in the hand, as a rule. Guinea-p
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. 226 Experimentation upon Animals When, in studying Pfeifier's phenomenon and similar conditions, it is desirable occasionally to withdraw drops of fluid from the ab- dominal cavity, a small opening can be burned through with a blunt needle. This does not heal readily, and through it, from time to time, a capillary pipet can be introduced and the fluids withdrawn. Small animals, such as rabbits and guinea-pigs, can be held in the hand, as a rule. Guinea-pig and rabbit-holders of various forms can be obtained from dealers in laboratory supplies. The best of these is undoubtedly that of Latapie, shown in the accompanying illustration. Dogs, cats, sheep, and goats can be tied and held in troughs. A convenient form of mouse-holder, invented by Kitasato, is shown in the figure. In all these experiments one must remember that the amount of material introduced into the animal must be in proportion to its size, and that injection experiments upon mice are usually so crude and destructive as to warrant the comparison drawn by Frankel, that the injection of a few minims of liquid into the pleural cavity of a mouse is "much the same as if one would inject through a fire-hose three or four quarts of some liquid into the respira- tory organs of a ; Method of Securing Blood from Animals.— For many experimental purposes it becomes necessary to secure blood in larger or smaller quantities from animals. For horses, cattle, calves, goats, sheep, large dogs, etc., this is a simple matter, all that is necessary being to restrain the animal, make a minute incision in the skin over the jugular vein, which is easily found by compressing it at the root of the neck and noting where the vessel expands, and in- troducing a canula when the vein is well dis- tended. The trocar being withdrawn, the blood at once flows. A sterile tube is slip
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Keywords: ., bookcentury1900, bookdecade1910, booksubjectbacteri, bookyear1916
