. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. DIPLOCOCCUS GONORRHCE^ 381 The gonococcus is non-motile and does not form spores. It is easily stained with the usual aqueous anilin dyes. Methylene-blue alone, or eosin followed by methylene-blue, or the neutral red stain of Plato,' gives good results. Gram's method of staining, however, is the only one of differential value. With this method the gonococcus is rapidly decolorized and can be counterstained with fuchsin or Bismarck brown. The Gram stain applied to pus from the male uret
. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. DIPLOCOCCUS GONORRHCE^ 381 The gonococcus is non-motile and does not form spores. It is easily stained with the usual aqueous anilin dyes. Methylene-blue alone, or eosin followed by methylene-blue, or the neutral red stain of Plato,' gives good results. Gram's method of staining, however, is the only one of differential value. With this method the gonococcus is rapidly decolorized and can be counterstained with fuchsin or Bismarck brown. The Gram stain applied to pus from the male urethra, while not absolutely reliable, is, for practical purposes, sufficiently so to make a diagnosis. In exudates from the vagina or from the eye the mor- phological picture is not so reliable, owing to the frequent presence. Fia. 80.—Gonorrheal Pus prom Urethra, showing the Cocci within a Leucocyte. in these regions of other Gram-negative cocci. The great scarcity of gonococci in very chronic discharges necessitates thorough cultural iavestigatibn; negative morphological examination in such cases can not be regarded as conclusive.^ Cultivation.—^The gonococcus is extremely delicate and is difficult to cultivate. After many failures to grow it upon the ordinary media, Bumm^ obtained his first growths upon human blood serum which had been heated to partial coagulation. The medium most commonly used at the present day was introduced ' Plato, Berl, klin. Woch., 1894. 'Heiman, Medical E-eqord, 1896. 3 Bumm, Deut. med. Woch., 1885,. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Hiss, Philip Hanson, 1868-1913; Zinsser, Hans, 1878-1940. joint author. New York and London, D. Appleton and Company
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