. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Cultivation 389 The cocci have also been cultivated from the nasal discharges in 6 cases studied by Weichselbaum, and in 18 studied by Scherer. Elser* has isolated the organism from the circulating blood of patients suffering from epidemic cerebro-spinal fever. To determine the presence of the coccus in the nasal discharges where other similar cocci may be present, Gram's stain may be used and followed by an aqueous solution of Bismarck-brown. The men- i
. A text-book upon the pathogenic Bacteria and Protozoa for students of medicine and physicians. Bacteriology; Pathogenic bacteria; Protozoa. Cultivation 389 The cocci have also been cultivated from the nasal discharges in 6 cases studied by Weichselbaum, and in 18 studied by Scherer. Elser* has isolated the organism from the circulating blood of patients suffering from epidemic cerebro-spinal fever. To determine the presence of the coccus in the nasal discharges where other similar cocci may be present, Gram's stain may be used and followed by an aqueous solution of Bismarck-brown. The men- ingococci will be Fig. 142.—Technic of spinal puncture. The patient is sitting on the edge of a chair and is bent forward; the crests of the ilia are indicated by black lines, and are on a level with the spinous process of the fourth lumbar vertebra; the "soft spot" is found just above. The first tube receives the first few drops of fluid, which are usually blood tinged. (Kolmer.) Cultivation.—The organism was successfully cultivated by Weichselbaum, but does not readily adapt itself to artificial media. It develops upon agar-agar and glycerin agar-agar^ upon Loffler's blood-serum mixture, and, according to Goldschmidt,t upon potato. Weichselbaum did not find that it developed upon potato. It does not grow in bouillon or gelatin. The cultures are usually scanty and without characteristic features. * "Jour. Medical Research," 1906, xiv, 89. t "Centralbl. f. Bakt. u. Parasitenk.," 11, 22, Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original McFarland, Joseph, 1868-. Philadelphia and London, W. B. Saunders Company
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