Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microoÌrganisms . ^The presence of B. coli in surface waters isnatural, owing to contamination with the fecal discharges ofman and other animals. In well-water its presence denotessewage or surface contamination, and such a well should becondemned until free from coli. (See Water Analysis.) Bacillus of Typhoid or Enteric Fever (Eberth-Gaffky).âOrigin.âEberth, in the year 1880, found this bacillus inthe spleen and lymphatic glands of persons d)dng of typhoid,and Gaffky isolated and cultiv
Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microoÌrganisms . ^The presence of B. coli in surface waters isnatural, owing to contamination with the fecal discharges ofman and other animals. In well-water its presence denotessewage or surface contamination, and such a well should becondemned until free from coli. (See Water Analysis.) Bacillus of Typhoid or Enteric Fever (Eberth-Gaffky).âOrigin.âEberth, in the year 1880, found this bacillus inthe spleen and lymphatic glands of persons d)dng of typhoid,and Gaffky isolated and cultivated the organism four yearslater. 136 ESSENTIALS OF BACTERIOLOGY Form.â^Rods with rounded ends about three times as longas they are broad. Usually solitary in tissue-sections, butin old artificial cultures found in long threads. Flagella onaU sides (Fig. 58). Properties.âVery motile. Spores have not been found;they do not liquefy gelatin. Growth.âThey are facultative anaerobic; grow best at 37°C, but can also develop at ordinary room temperature. Theydevelop chiefly on the surface, and very slowly. Repeated. Fig. 58.âBacillus typhi, from an agar-agar culture six hours old,showing the flagella stained by Lofflers method (Xiooo) (Frankel andPfeiffer). freezing and thawing do not affect the vitaHty of the germ,and phenol in i to 2 per cent, solution has no effect on ten-minute exposure to 60° C. is invariably fatal. Colonies on Gelatin Plates.â^Two forms: the ones near thesurface spread out like a leaf, transparent, with bluish fluor-escence. The deeper ones resemble whetstone crystals ofuric acid, with the same yellowish tinge (Fig. 59). In five days they attain to 3 millimeters in diamete*-. THE COLON-TYPHOIB GROUP 137 Bile Salt Media.âRapid growth without gas formation; anumber of special media suited for the growth of typhoid,namely, Jacksons, Hesse, Hiss, Conradi-Drigalski, etc. (SeeWater Analysis and formula for Media.) Stab-cultures.âMainly on the surf
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