. Dourine of horses : its cause and suppression. the edematous swellings spread, involving the whole inner surface ofthe thighs, as well as the entire length of the sheath, the appetitebecame poor, and the animal died in November, about 6 weeks afterits arrival or 8 weeks after the inoculation. During the life of thedog after the first appearance of symptoms microscopic examinationswere made daily of the blood taken from the peripheral circulationas well as from the serum in the edematous areas about the genitalsfdr the purpose of recording the trypanosomes present, but theywere always extreme
. Dourine of horses : its cause and suppression. the edematous swellings spread, involving the whole inner surface ofthe thighs, as well as the entire length of the sheath, the appetitebecame poor, and the animal died in November, about 6 weeks afterits arrival or 8 weeks after the inoculation. During the life of thedog after the first appearance of symptoms microscopic examinationswere made daily of the blood taken from the peripheral circulationas well as from the serum in the edematous areas about the genitalsfdr the purpose of recording the trypanosomes present, but theywere always extremely scarce. After death these organisms werefound in great numbers in the heart, blood, lungs, hver, kidneys,and spleen. It was with the trypanosomes obtained from this dog, kept aUveby continued passage through white rats, rabbits, dogs, and horses,that most of our work with the organism has been done. Whiterats were by far the most satisfactory experiment animals for the Bui. 14a, Bureau of Animal Industry, U. S. Dept. of Agriculture. Plate Fig. 2.—Trypanosoma Equiperdum from a Culture. CAUSE. 13 propagation of the organism, as it proved luuch more pathogenicfor them, death usually occurring in from 2 to 6 months afterinoculation. The trypanosomes were also more readily recoveredfrom these animals. Although rabbits after inoculation became grad-ually emaciated and finally died in from 2 to 8 months, it was onlyby long and careful microscopic examination that any trypanosomescould be found in their viscera after death. The length of time which this protozoan lives when kept in bloodor body fluids taken from the dead animals varies considerably, butis generally stated as being a very short time. Thus Rouget observedit to keep its motihty for 18 hours, but never after 24 hours. In theblood of the French dog, as well as in physiologic salt solution emul-sions of the liver and spleen, the parasites were actively motile onthe second day, a few were still observed on the third
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