. Pharmaceutical bacteriology. Bacteriology; Disinfection and disinfectants. 174 PHARMACEtTTICAL BACTERIOLOGY 1 A second and later improvement in the method of inoculating seeds with root tubercle bacteria (Rhizobia) is given by Hartleb in the specifications forming part of letters patent No. 674,765, granted May 21,1901, at Wash- ington, D. C. Although not so stated in the specifications, it is evident that the Hartleb process is a method for applying pure rhizobia cultures to seed of leguminous plants. Whether the method offers any advantages over the method of Nobbe and Hiltner is questiona
. Pharmaceutical bacteriology. Bacteriology; Disinfection and disinfectants. 174 PHARMACEtTTICAL BACTERIOLOGY 1 A second and later improvement in the method of inoculating seeds with root tubercle bacteria (Rhizobia) is given by Hartleb in the specifications forming part of letters patent No. 674,765, granted May 21,1901, at Wash- ington, D. C. Although not so stated in the specifications, it is evident that the Hartleb process is a method for applying pure rhizobia cultures to seed of leguminous plants. Whether the method offers any advantages over the method of Nobbe and Hiltner is questionable. In any case it would prove practically advantageous only under the conditions referred to under the discussion of nitragin. Although the method has been freely discussed and experimented upon in Germany, the fertihzer is no longer. Fig. S4-—R- mutabile as it appears in mature nodules of red and white clover root nodules. This may be considered the extreme form variation due to hyper- nutrition. on the market. There is on the market a third patented germ or microbe soil fertilizer of German origin, knoTvn as "; It consists essentially of a pure culture of the soil bacillus known as Bacillus ellenhachiensis- alpha or Bacillus ellenhachiensis Caron. The germ was first brought tO' the attention of the agriculturists by Caron, a land owner of Germany,, who first isolated it and called attention to the fact that it had the power of chemically binding the free nitrogen of the air. The microbe is said to be closely alhed to B. megatherium and B. suhtilis. According to some authorities it is especially concerned in assimilating free nitrogen for gramineous plants. If it is true it may prove of great value to grain growers. The commercial alinit is a dry pulverulent substance of a yellowish- gray color, with about 10 per cent, moisture and per cent, nitrogen. It is evidently prepared by mixing spore-bearing pure cultures of the bacillus. Please note that these
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