. California fish and game. Fisheries -- California; Game and game-birds -- California; Fishes -- California; Animal Population Groups; Pêches; Gibier; Poissons. .).( C VLIFORN IA FIS11 AND GA M E The salinity !' our artificial environments changed from roughly three-quarters \ C) to approximately double (A - C) thai of sea water with only a \ to \ C clump' in ihc blood concentration of the killifish I Figure 1 . As the salinity decreases from \ [.25 C, the freezing poinl of the blood decreases fairly rapidly in comparison with the osmoregulatory plateau between \


. California fish and game. Fisheries -- California; Game and game-birds -- California; Fishes -- California; Animal Population Groups; Pêches; Gibier; Poissons. .).( C VLIFORN IA FIS11 AND GA M E The salinity !' our artificial environments changed from roughly three-quarters \ C) to approximately double (A - C) thai of sea water with only a \ to \ C clump' in ihc blood concentration of the killifish I Figure 1 . As the salinity decreases from \ [.25 C, the freezing poinl of the blood decreases fairly rapidly in comparison with the osmoregulatory plateau between \ and \ *'. Initially the change is quite rapid bu1 it soon levels off. The change for blood in this pari of the curve is \ C for an environ- mental change of \ C. The osmoregulatory curve never reaches <'. The discrepancj bet ween and \ C. is apparently due to the incomplete replacemenl of salt water and the extreme "hardness" of (lie tap water in Santa Barbara. oo. < o AC ENVIRONMENT FIGURE 1— Osmoregulatory curve for California killifish, Fundulus parvipinnis. Note the ex- tensive plateau, which probably corresponds to this fish's normal environmental salinity range. Perhaps the most interesting portion of the curve is between and C. Between these salinities there is a rapid loss of osmoregulatory ability; the freezing point of the blood is de- pressed from A -- to A - - C. At this particular point only two fish remained alive in the hypersaline tank. This, of course, indicated a very rapid breakdown in osmoregulatory ability due to overtaxing the normal channels of salt excretion and secretion, to drastically inter- fering with metabolic processes, or to both. DISCUSSION The California killifish is admirably equipped to survive in its brack- ish habitat, Goleta Slough, where our specimens were caught, is a shallow body of estuarine water averaging 2 to 3 ft deep at mean low tide. The slough receive


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