. A treatise on Asiatic cholera. Fig. 5.—Colonies of comma-bacilli growing on the gelatine-plate. Magnified 80 times. When the colony becomes somewhat larger, this granulation becomes moreand more evident; at last it looks like a little heap of strongly refracting might best compare the appearance of such a colony with the appearance of alittle heap of pieces of glass. As they grow, the gelatine liquelies in the immedi-ate neighborhood of the bacteria colony, and this latter at the same time sinksdown deeper into the mass of gelatine. A funnel-shaped cavity is thus formed inthe gela
. A treatise on Asiatic cholera. Fig. 5.—Colonies of comma-bacilli growing on the gelatine-plate. Magnified 80 times. When the colony becomes somewhat larger, this granulation becomes moreand more evident; at last it looks like a little heap of strongly refracting might best compare the appearance of such a colony with the appearance of alittle heap of pieces of glass. As they grow, the gelatine liquelies in the immedi-ate neighborhood of the bacteria colony, and this latter at the same time sinksdown deeper into the mass of gelatine. A funnel-shaped cavity is thus formed inthe gelatine, in the midst of which the colony is seen as a little whitish appearance is also qvnte peculiar. It is seen, at least in this manner, in veryfew other kinds of bacteria, and, as far as I know, never so marked as with thecomma-bacilli. The sinking of the colonies can be best observed when carrying. Figs. 0 and 7. Fig. 6.—Funnel-shaped sinking in the gelatine at the inoculation point in the 7.—Natural size of the colonies as they appear on the gelatine plate. out an artificial cultivation. A suitable colony is selected on the gelatine plate,under a microscope with a low power; it is touched with a platinum wire,previously heated; the bacilli are transferred by the wire into a test-tube withgelatine, and this is closed with sterilized wadding. A cultivation of this kindthen grows in the same manner as the colony on the gelatine-plate. I am in pos- 156 ASIATIC CHOLERA. session of a nuniorous collection of artificial cultivations of bacteria, made in thismanner; but I have never seen in their case sucli as the comma-bacillicause after being- transferred into the g-elatine. Here, also, as soon as the culti-vation begins to develop, you see a little funnel, which marks the point where tlieinocuUition took place. By deg-rces, the gelatine liquefit^s in the
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Keywords: ., bookcentury1800, bookdecade1880, bookpublishernewyo, bookyear1885
