. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. I 0 5 SULFIDE EQUILIBRATION (WIN) 10 0 10 BLOOO EQUILIBRATION (WIN) FIGURE 4. Time-dependent effects of sulfide on Riftia pachyptila plume CytOx activity, and time- dependent reversal of inhibition by blood. Sulfide was added to CytOx preparations, and activity was measured at the times indicated. The filled circles represent time of pre-incubation of enzyme preparations with sulfide prior to initiation of assay. The open diamond symbols represent addition of 10 /xl of blood at t = 0, and immediate assay. Open circles repres
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. I 0 5 SULFIDE EQUILIBRATION (WIN) 10 0 10 BLOOO EQUILIBRATION (WIN) FIGURE 4. Time-dependent effects of sulfide on Riftia pachyptila plume CytOx activity, and time- dependent reversal of inhibition by blood. Sulfide was added to CytOx preparations, and activity was measured at the times indicated. The filled circles represent time of pre-incubation of enzyme preparations with sulfide prior to initiation of assay. The open diamond symbols represent addition of 10 /xl of blood at t = 0, and immediate assay. Open circles represent addition of blood after 10 min pre-incubation of enzyme preparations with sulfide; assays were then initiated 0, 5, and 10 min after blood addition. Filled squares represent CytOx activity of enzyme preparations without sulfide added. of sulfide reduced or eliminated the ability of the blood to protect CytOx activity, indicating a saturation of the sulfide-binding capacity of the blood at higher sulfide concentrations. The finding that the protective effects of blood could be reduced or 100. 0 SULFIDE in BLOOD (mM) FIGURE 5. Effects of pH and blood sulfide concentration on protection by blood of CytOx activity. Ten n\ of blood containing known amounts of sulfide were added to CytOx assays inhibited by 2 pM sulfide (pH ), and 10 pM sulfide (pH ). Sulfide-free blood (10 n\) provided 100% protection (equal to control activity without sulfide added) under the assay conditions Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biol
Size: 1826px × 1369px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
