. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 346 S. SUZUKI I • SCO - ,00 £ 4OO O TJ a, 3OO o O 200 100. :x i_ o o 100 \ 80 60 tf) 0) o o o t Ov-i 10 t RI 20 t St 30 EC 40 0) .a E 3 20 Z EAJ3] Molting stages Days Ov-2 FIGURE 1. Oocyte growth during the molting cycle following first oviposition in Armadillidium vul- gare. — O —, diameter of oocyte;-- • --, number of oocytes; Ov-1, first oviposition; Ov-2, second oviposi- tion; RI, release of larva; St. sternoliths; EC, ecdysis. Ventrical lines around the points show standard errors. MATERIALS AND METHODS Animals Armadill
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 346 S. SUZUKI I • SCO - ,00 £ 4OO O TJ a, 3OO o O 200 100. :x i_ o o 100 \ 80 60 tf) 0) o o o t Ov-i 10 t RI 20 t St 30 EC 40 0) .a E 3 20 Z EAJ3] Molting stages Days Ov-2 FIGURE 1. Oocyte growth during the molting cycle following first oviposition in Armadillidium vul- gare. — O —, diameter of oocyte;-- • --, number of oocytes; Ov-1, first oviposition; Ov-2, second oviposi- tion; RI, release of larva; St. sternoliths; EC, ecdysis. Ventrical lines around the points show standard errors. MATERIALS AND METHODS Animals Armadillidium vulgarewas collected near Yokohama in early March 1986. Sev- eral females (10-12 mm body length) were kept in a petri dish with moistened soil. Two adult males were added to the dish for a normal reproductive cycle. They were maintained at 25 ± 2°C in natural daylight, and fed decayed leaves and rat chows. To observe oocyte growth, fresh ovaries were examined microscopically. Preparation of hemolymph and ovarian homogenate to electrophoresis Hemolymph samples were collected using a capillary tube placed in a hole in the tergite of the seventh thoracic segment. It was diluted 1/10 with Tris-HCl buffer (20 mM, pH containing 130 mM NaCl and 5 mM EDTA). Dissected ovaries were rinsed and homogenated in 300 ^1 Tris-HCl buffer. Ovarian homogenate was passed through prefilter (milipore, AP) to remove lipids, centrifuged at 20,000 X g for 15 min at 4°C, and the supernatant was collected as a sample of ovarian homogenate. Polyacrylamide gel electrophoresis Polyacrylamide gel electrophoresis (PAGE) was performed using 5% acrylamide monomer (Davis, 1964). Polyacrylamide gel electrophoresis in sodium dodecyl sul-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Ho
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
