. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. INLAND CULTURE OF A NUDIBRANCH 245. B vacuum MFSA l I D i *aftU$] juvenile larval experiments /K egg experiments mas ses T adult air V f \ J larvae experments juveniles /N 1 1 adults \\E , / > , \\ 0 F \- L G L° larvae "•>:r juveniles 1 \\ > Cj* [j^^t ^ adults Figure 1. Algorithm for the culture of Hcrxhui trn (A) Aquaria with undergravel filtration and a 3-4 inch thick bed of CaCO, gravel are filled with freshly prepared artificial seawater. A few inverte- brates or fish are added to each aquarium aft
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. INLAND CULTURE OF A NUDIBRANCH 245. B vacuum MFSA l I D i *aftU$] juvenile larval experiments /K egg experiments mas ses T adult air V f \ J larvae experments juveniles /N 1 1 adults \\E , / > , \\ 0 F \- L G L° larvae "•>:r juveniles 1 \\ > Cj* [j^^t ^ adults Figure 1. Algorithm for the culture of Hcrxhui trn (A) Aquaria with undergravel filtration and a 3-4 inch thick bed of CaCO, gravel are filled with freshly prepared artificial seawater. A few inverte- brates or fish are added to each aquarium after 3-7 days of operation. After one month of operation, additional anemones or other animals are added to the aquaria. Salinity is adjusted each week. Water from these aquaria is designated as "seasoned aquanum ; (B) Seasoned aquarium water is regularly removed from established aquana and used lor egg mass, larval, juvenile and adult cultures. (C) Seasoned aquarium water is filtered through a ^m Millipore filter for culture use. (MFSA: Millipore-filtered seasoned aquarium water.) (D) Adults for stock cultures are maintained in small bowls (about 13 cm diameter, 5 cm deep). Bowl and water are changed daily. Appropriately sized anemone prey are added as needed. (E) Egg masses are collected from adult cultures and placed in 500-ml beakers containing —350 ml of MFSA. Aeration is provided through Pasteur pipettes. Beaker and water are changed daily. As egg masses hatch, larvae are concentrated with a Nitex filter (see Switzer- Dunlap and Hadfield. 1981, p. 208) and used in larval experiments or metamorphosis cultures. (F) For metamorphosis cultures, larvae are placed in crystallizing dishes containing MFSA and a few small anemones. Water is left unchanged for 5-10 days and then the water is changed daily, and the bowl every few days as needed, until juveniles are large enough to transfer to finger bowls (2-3 weeks after metamorphosis). Juveniles are harvest
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
