Practical physiological chemistry; a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . trate have been formed. Theformer is insoluble in the cold solution and separates on standing. After standingseveral hours filter off the hypoxanthine silver nitrate and wash with water untilthe wash water is only slightly acid in reaction. Examine the crystals of hypo-xanthine silver nitrate under the microscope and compare them with thoseinFig. 84, above. Now wash the crystals from the paper into a beaker with a Uttlewater and warm the liquid. Rem
Practical physiological chemistry; a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . trate have been formed. Theformer is insoluble in the cold solution and separates on standing. After standingseveral hours filter off the hypoxanthine silver nitrate and wash with water untilthe wash water is only slightly acid in reaction. Examine the crystals of hypo-xanthine silver nitrate under the microscope and compare them with thoseinFig. 84, above. Now wash the crystals from the paper into a beaker with a Uttlewater and warm the liquid. Remove the silver by hydrogen sulphide and filter. ^ For an improved method of preparing pure creatine from creatinine see chapter onPhysiological Constituents of the Urine.^ Walpole: Jour. Physiol., 42, 301, 1911. MUSCULAR TISSUE 351 By this means hypoxanthine nitrate has been formed and is present in the filtrate.(For crystalUne form of hypoxanthine nitrate see Fig. 40, p. 136.) Concentrateon a water-bath to drive ofif hydrogen sulphide, and render the solution sUghtlyalkaUne with ammonia. Warm for a time, to remove the free ammonia, Fig. 112.—Hypoxanthine Silver Nitrate.(Drawn from a student preparation by Dr. E. F. Hirsch.) concentrate the filtrate to a small volume and allow it to stand in a cool should crystaUize in small colorless needles. Examine thecrystals under the microscope. 3. Xanthine.—To the filtrate from the above experiment containing the
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