Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . n. It consists of a special gradu-ated tube (A), a blood pipet (B) which holds 20 cmm.(15 mg. by weight) of blood, a reagent pipet (D), and ahand spectroscope (E). The method of using this instrument is as follows:The tube (A), into which fits the pipet (B), is held hori-zontally in such a manner that the pipet may be filledby capillary attraction with blood from the finger or a medicine dropper, containing distilled w^ater, thepipet is washed free of blood, using sufl5cient w
Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . n. It consists of a special gradu-ated tube (A), a blood pipet (B) which holds 20 cmm.(15 mg. by weight) of blood, a reagent pipet (D), and ahand spectroscope (E). The method of using this instrument is as follows:The tube (A), into which fits the pipet (B), is held hori-zontally in such a manner that the pipet may be filledby capillary attraction with blood from the finger or a medicine dropper, containing distilled w^ater, thepipet is washed free of blood, using sufl5cient water tobring the diluted blood to the zero-point of the tube diluted blood is then thoroughly mixed by shakingand inverting the tube, after which agitation it is allowedto settle. In this process care should be taken to closethe opening (C) with the thumb to prevent loss of reagent pipet (D) is then filled with the following test solution (N/200tartaric acid, each of which is equivalent to .0002 gram of NaOH) and isconnected with the end of the blood pipet by means of rubber Fig. 120.—Dareshemoalkalimeter: A,alkalimeter tube; B,automatic blood pi-pet; C, air hole; D,reagent pipet. Tartaric acid,Alcohol (95 per cent.),Distilled water, q. s. ad. , By compression of the bulb of the pipet the reagent is forced through theblood pipet into the tube A. Mix the fluids by inversion, care being takento avoid loss of fluid. Adjust the spectroscope to the tube below the zeropoint after each addition of reagent and obsen^e the spectrum for the presence iPhila. Med. Joiur., 1903, XI, 137. * Centralbl. f. d. med. Wissensch., 1898, XXXVI, 913. I THE BLOOD. 397 of the two bands of oxyhemoglobin. This is best done by holding the tube aconstant distance from an artificial light. This part of the technic is verytedious, but is necessar} until the observer learns to associate the change incolor of the mixture with the point of neu
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