. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 142 ROMANOFF AND HAYWARD After the amniotic membrane is pierced, any escaping liquid can be collected directly from the dish. On the whole, this method permits quick and accurate measurements of the fluids and their ready use for further immediate studies. The specific gravity of the fluids was determined by weighing them in small standardized pycnometers of approximately cc. capacity. These pycnometers were made in 14 mm. lengths from glass tubing with a 3 mm. bore. In a test determination by this method the average dev
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 142 ROMANOFF AND HAYWARD After the amniotic membrane is pierced, any escaping liquid can be collected directly from the dish. On the whole, this method permits quick and accurate measurements of the fluids and their ready use for further immediate studies. The specific gravity of the fluids was determined by weighing them in small standardized pycnometers of approximately cc. capacity. These pycnometers were made in 14 mm. lengths from glass tubing with a 3 mm. bore. In a test determination by this method the average deviation of density was about The hydrogen-ion concentration (pH) of the fluids was measured electro- metrically, using a hydrogen electrode of special design for small quantities of material of about cc. EXPERIMENTAL RESULTS Volume of fluids under normal conditions The actual volume of allantoic and amniotic fluids in the eggs of the various species showed enormous variation (Figures 1 and 2), since the weight of the eggs used varied from about 9 grams for quail to about 85 grams for turkey. cc. 10 8 Li. y o i- FIG. SPGR. FIG. 3. CHICKEN PHEASANT QUAIL - TURKEY DUCK GOOSE PH 9 FIG. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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