. Laboratory work in bacteriology. Bacteriology. GELATIN AND POTATO MEDIA. 155 For practical purposes it is sufficient to dissolve 40 g. of sodium hydrate in distilled water, and to dilute this solution to one liter. This will give an approximately normal solu- tion. 2.âOne that will contain 4 g. of this base in one liter of water. This is known as a deci-normal (^) sodium hydrate solution. It is prepared by taking 100 of the normal solution, and diluting this with distilled water to one liter. It is evident that this solution has one-tenth the strength of the former. These two solutions
. Laboratory work in bacteriology. Bacteriology. GELATIN AND POTATO MEDIA. 155 For practical purposes it is sufficient to dissolve 40 g. of sodium hydrate in distilled water, and to dilute this solution to one liter. This will give an approximately normal solu- tion. 2.âOne that will contain 4 g. of this base in one liter of water. This is known as a deci-normal (^) sodium hydrate solution. It is prepared by taking 100 of the normal solution, and diluting this with distilled water to one liter. It is evident that this solution has one-tenth the strength of the former. These two solutions are placed in the apparatus shown in Fig. 24. This consists of two burettes, each of 50 capa- city, graduated in iV , and connected with bottles or flasks which contain the nor- mal and decinormal solutions, respectivelv. ^"'' ^^' ^"^^''^^ ^°^ titrating nutrient '^. â ^' media. 3âConnected with stock of ^NaOH Titration of the aelatin.âBv ^°l"'io°- *âConnected with stock of . â ' N NaOH solution (F. ). means of a pipette measure out 10 of the gelatin solution into each of four test- tubes, and label these 1, 2, 3, and 4. To tube 1 add of the ft NaOH solution. To tube 2 add of the ft NaOH solution. To tube 3 add of the ft NaOH solution. To tube 4 add of the ft NaOH V Heat each tube in the flame till the liquid boils, o order to expel carbonic acid, and to precipitate phosphat-f-^ and albumin. Then, drop into each tube a strip of blue. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Novy, Frederick George, 1864- . Ann Arbor [Mich. ] G. Wahr
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