. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 184 M. YONEDA AND S. NEMOTO. Figure 1. Unfertilized eggs stained with DAPI. Three eggs in the upper row are binucleates. One egg in the lower left is trinucleate. Two eggs (central and right in the lower row) are mononucleates. Scale bar = 100 ^m. center of the egg. No difference was detected between the time course of a single nucleus and that of each of partial nuclei. According to Hamaguchi and Hiramoto (1986) two partial pronuclei fuse and then fuse with the sperm nucleus. Fused zygote nuclei formed a spindle (Fig. 3), a
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 184 M. YONEDA AND S. NEMOTO. Figure 1. Unfertilized eggs stained with DAPI. Three eggs in the upper row are binucleates. One egg in the lower left is trinucleate. Two eggs (central and right in the lower row) are mononucleates. Scale bar = 100 ^m. center of the egg. No difference was detected between the time course of a single nucleus and that of each of partial nuclei. According to Hamaguchi and Hiramoto (1986) two partial pronuclei fuse and then fuse with the sperm nucleus. Fused zygote nuclei formed a spindle (Fig. 3), and the time of the first cleavage was normal. Thus, partial nuclei can only be detected during a short interval of de- velopment, and they leave no noticeable trace of their occurrence. Von Ledebur-Villeger (1972) and Mar (1980) found that the central migration of female nuclei still occurs, even in parthenogenetically activated sea urchin eggs without sperm asters. We confirmed this in Clypeaster. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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