. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. A B C D E F Figure I. Autoradiogram of immunoprecipitates from oocyte extracts shows Ku-like polypeptides. Stage 5 oocytes were radiolabeled with [15S]-methiomne (see Materials and Methods) by incubating the oocytes for 18 h at 18°C. Ten oocytes were used for each immunoprecipitation assay, (a) Ku (p80/p70) immunoprecipitated with a monoclonal antibody (mAb 1621 from HeLa cell extract (A); oocyte extracts immunoprecipitated with normal human serum (B). human autoimmune serum (KT) having anti-Ku antibody (C). human autoimmune
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. A B C D E F Figure I. Autoradiogram of immunoprecipitates from oocyte extracts shows Ku-like polypeptides. Stage 5 oocytes were radiolabeled with [15S]-methiomne (see Materials and Methods) by incubating the oocytes for 18 h at 18°C. Ten oocytes were used for each immunoprecipitation assay, (a) Ku (p80/p70) immunoprecipitated with a monoclonal antibody (mAb 1621 from HeLa cell extract (A); oocyte extracts immunoprecipitated with normal human serum (B). human autoimmune serum (KT) having anti-Ku antibody (C). human autoimmune serum having anti-Ro antibody (D). and human autoimmune serum having anti- Sm antibody (E). (b) Radioimmunoprecipitates of oocyte extracts with pre-immune IgG (A'), mAb 162 (B'). and with human autoimmune sera having anti-Ku antibodies; serum OM (C'), serum HT (D'l, serum KT (E'l, serum KY (F'l. (c) Autoradiogram of immunoprecipitates from oocyte extracts shows Ku-like polypeptides during oogenesis. The OM serum, containing the anti-Ku antibody, was used for the immuno- precipitation assay. The number of oocytes for each stage was standardized so as to use equal amounts of total protein for each immunoprecipitation: Stage I (Yl) (Lane A), stage II (Y2) (Lane B), stage III (Y3) (Lane C), stage IV (Y4) (Lane D), stage V (Y5) (Lane E). and stage VI (Y6) (Lane F). The Ku polypeptides (p80 and p70) are indicated (arrows). Another protein band running slightly faster than p80 (*) is not identified, but may be a degradation product of p80. The unidentified band is not immunoprecipitated by mAb 162 or other autoimmune sera that recognize Ku (Fig. Ib). (lanes D-E). We have not determined if the increased synthesis observed in the later stages of oogenesis corre- lates with a stage-specific function or if the oocytes stock- pile DNA-PK for use after fertilization. The immunopre- cipitated Ku is not as abundant in Y6 oocytes as in Y5 oocytes. This difference may be due to the decreased
Size: 3365px × 743px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
