. Essentials of laboratory diagnosis; designed for students and practitioners. cture of the skin made with aGlover needle, the half-point of a new steel pen, a Dalandlancet (see Fig. 9), or the so-called pistol-knife. The last-mentioned two instruments are to be preferred because theypermit of regulation of the depth of the puncture. For hospital and laboratory use, in order to assure a sterileinstrument for each patient, I have, for a number of years,employed short sections of thin glass tubing, drawn out in theflame to a capillary point, and then broken so as to have ashort, sharp end. There
. Essentials of laboratory diagnosis; designed for students and practitioners. cture of the skin made with aGlover needle, the half-point of a new steel pen, a Dalandlancet (see Fig. 9), or the so-called pistol-knife. The last-mentioned two instruments are to be preferred because theypermit of regulation of the depth of the puncture. For hospital and laboratory use, in order to assure a sterileinstrument for each patient, I have, for a number of years,employed short sections of thin glass tubing, drawn out in theflame to a capillary point, and then broken so as to have ashort, sharp end. There is little danger, if these are properly 4 50 THE BLOOD. made, of leaving fragments of glass in the tissues. Afterusing, these may either be thrown away or retipped in theflame. For larger quantities of blood, a few cubic centimeters ormore, it is advisable, provided there is no contraindication, toobtain the specimen by the use of wet cups. It must be bornein mind that by this method there is always more or less ad-mixture of lymph. Another method is to draw the blood from. -Fleischl Hemoglobinometer, surrounded by accessoriesnecessary to performance of the test,including daland lancet. a dilated vein into a large sterile antitoxin syringe. Lastly, anordinary venesection may be resorted to. The withdrawal of blood, if aseptically performed, is prac-tically free from danger and need disturb the patient very proceeding in any case it is advisable to determine theabsence of hemophilia in the patient. CLINICAL METHODS. The clinical methods commonly applied to the blood in thestudy of disease are: The estimation of the percentage of ESTIMATION OF THE 11KMOGLOBIN. 51 hemoglobin; the enumeration of the erythrocytes and the whiteblood-corpuscles; a differential count of the various white ele-ments: estimation of the coagulation time and of the viscosity. The apparatus necessary for the performance of these sev-eral examinations are as follows:— 1. A good microscope wit
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