. The microscope; an introduction to microscopic methods and to histology. Microscopes. 394 STAINING MICROSCOPIC OBJECTS [Ch. XI § 639. Staining with hematoxylin. âTake a slide of sections pre- pared by the paraffin or the collodion method from the jar of alcohol and plunge it into a vessel of water to remove the alcohol. For stain- ing put the shde of sections into a jar or shell vial of the hematoxylin solution or one can lay the shde flat on the staining rack or some other support and add the stain to the sections (fig. 235-236). It usually takes from 2 to 10 minutes to stain sufficiently
. The microscope; an introduction to microscopic methods and to histology. Microscopes. 394 STAINING MICROSCOPIC OBJECTS [Ch. XI § 639. Staining with hematoxylin. âTake a slide of sections pre- pared by the paraffin or the collodion method from the jar of alcohol and plunge it into a vessel of water to remove the alcohol. For stain- ing put the shde of sections into a jar or shell vial of the hematoxylin solution or one can lay the shde flat on the staining rack or some other support and add the stain to the sections (fig. 235-236). It usually takes from 2 to 10 minutes to stain sufficiently with hematoxylin. A good plan when one is learning the .w^^.«.^^xx^^-v\ process is to wash oS the stam after one ^i^^^^^ V â X minute, either with " >^Sia^â' ^f~3l fll^^^^^ 3, pipette or by put- tmg the shde m a dish of water. Wipe ^(i off the bottom of the â¢â "â¢'"'ai^^^^^i^E^^HW^BFJHB!^*"* slide and put it un- der the microscope. '"'â¢^"'"â - ^ Light well, use a low ^^.Ei-=^^aA**ss«. power, and one can vfy i see the nuclei stained , ,,,,â¢,,,,mu»iiiiraiii,im»imraiiiiiimmimtmâ¢immiii,iiiiii,iiiiiiiri,H,^/.i â ^ blulsh Or pUTple '''â ''*|;;.i i ' I ' color, as hematoxy- '' hn is a nuclear dye. Fig. 23s. Bowl with Draining Rack and Funnel jf .-. 1 ⢠r -^ FOR Staining Sections. " ^'^^ *^°^*^'^ ^^ ^^^^^' continue the stain- ing until the nuclei stand out boldly. Sometimes it takes a long time to stain well with hematoxyhn. In such a case the jar of stain may be put into the paraffin oven and the heat will accelerate the staining. One may also heat the individual slides as for spreading sections, but one must be careful not to let the stain dry on the sections. As the stain evaporates add fresh stain with a pipette. When the sections are well stained with hematoxyhn, wash off the hematoxylin with water. If the slide is allowed to stand some time in ordinary water the color is hkely to be brighter. This
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