. Ciba Foundation Symposium on the Regulation of Cell Metabolism. Cell metabolism. 60 E. C. Slater and W. C. Hulsmann and a sharp break in the rate when all the ADP is phos- phorylated. These experiments were carried out manometric- ally with reaction mixtures containing Mg2+. The rapidity with which the new rate of respiration sets in after the addition of ADP and the sharpness of the "cut-off" when all. 20 25 TIME (MINS.) Fig. 1. Respiratory control in rat liver mitochondria. Reaction mixture: phosphate, pH 7-4, 0-016 m; NaF, 0-012 m; MgClg, 0-006 m; KCl, 0-083 m; ATP, 10"* m;
. Ciba Foundation Symposium on the Regulation of Cell Metabolism. Cell metabolism. 60 E. C. Slater and W. C. Hulsmann and a sharp break in the rate when all the ADP is phos- phorylated. These experiments were carried out manometric- ally with reaction mixtures containing Mg2+. The rapidity with which the new rate of respiration sets in after the addition of ADP and the sharpness of the "cut-off" when all. 20 25 TIME (MINS.) Fig. 1. Respiratory control in rat liver mitochondria. Reaction mixture: phosphate, pH 7-4, 0-016 m; NaF, 0-012 m; MgClg, 0-006 m; KCl, 0-083 m; ATP, 10"* m; ethylenediaminetetra-acetate, 0-001 m; glutamate (dl), 0-007 m; sarcosomes, 0-6 mg. protein/ml. Reaction vol., 1 ml.; temp. 25°. Phosphate acceptor (a mixture of AMP and ADP) was added at the arrow. The measurement of Oj uptake com- menced 8 min. after the beginning of the experiment. The P/0 ratio, calculated from the amount of Og required to phosphorylate the amount of phosphate acceptor added, was 2-60. the ADP is phosphorylated are shown more clearly in the experiments of Chance and Williams (1955), who followed the change of oxygen concentration with an oxygen electrode. The requirement of ADP and inorganic phosphate for intracellular respiration can be accounted for by the following formulation of oxidative phosphorylation, which is an elaboration of that suggested earlier (Slater, 1953). Indeed, the elaborations are unnecessary to explain the effect of ADP. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Ciba Foundation Symposium on the Regulation of Cell Metabolism (1958 : London, England); Wolstenholme, G. E. W. (Gordon Ethelbert Ward); O'Connor, Cecilia M. (Cecilia Mary), 1927-. Boston, Little, Brown
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