. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 20 P. S. REDDY AND B. KISHORI. Time after injection (h) Figure 2. Time course of methionine-enkephalin-induced hyperglyce- mia. After injection of methionine-enkephalin (10~7 moL/crab). hemo- lymph was withdrawn from intact crabs at the time points indicated for glucose determination. Each point represents a mean ± SD (n = 10). Numbers in parentheses represent percent change from zero time controls. * Significant difference from zero time control at P < ** Signif- icant difference from zero time control at P < 0


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 20 P. S. REDDY AND B. KISHORI. Time after injection (h) Figure 2. Time course of methionine-enkephalin-induced hyperglyce- mia. After injection of methionine-enkephalin (10~7 moL/crab). hemo- lymph was withdrawn from intact crabs at the time points indicated for glucose determination. Each point represents a mean ± SD (n = 10). Numbers in parentheses represent percent change from zero time controls. * Significant difference from zero time control at P < ** Signif- icant difference from zero time control at P < NS Not significant from zero time control. kephalin, indicating conversion of inactive to active phos- phorylase. Effects of bilateral e\estalk ablation and injection of methionine-enkephalin into ablated crabs on carbohydrate metabolism Bilateral eyestalk removal caused a significant decrease in hemolymph carbohydrate level (Table 1). Enhancement of TCHO level of hepatopancreas and muscle was also significant in eyestalk-ablated crabs (Tables 2, 3). The in- crease was greater in muscle. Glycogen level in hepatopan- creas increased significantly in eyestalkless crabs. A similar pattern was observed in muscle. Tissue phosphorylase ac- tivity levels decreased significantly in eyestalk-ablated crabs (Tables 4, 5). Injection of methionine-enkephalin into eyestalkless crabs did not significantly change hemolymph carbohydrate levels compared to Ringer-injected eyestalkless crabs (Ta- ble 1). The levels of tissue TCHO and glycogen and activity levels of total and active phosphorylase were also not sig- nificantly altered in eyestalkless crabs after methionine- enkephalin injection (Tables 2-5). after the injection of methionine-enkephalin (Tables 4. 5). The ratio of active to total phosphorylase also increased in the tissues of crabs after the injection of methionine-en- Discussion The effect of eyestalk hormones on tissue carbohydrate levels and phosphorylase activity has been


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology