Archive image from page 32 of The development of a cosmid. The development of a cosmid map of chromosome 12p13 . developmentofcos00belk Year: 1998 CM rS U5 A < O O CJl CM «2 -cM''-''-''=''=' Ol T- O 1â CD OOinOiCMCOVOgpCM y T j-h j i_ /> «- CMcMCMrcMCMCMCMCMOJjicM. \ C123D6 â â ,-'' \ sisS Figure 5. STS map of Chromosome 12pl3. The sixteen markers, listed along the top of the figure, are being used in the current research. Five cosmids are shown mapped to their respective markers within the lower portion of the figure. (Davies, Jeffress Grant progress report 1996). Producti
Archive image from page 32 of The development of a cosmid. The development of a cosmid map of chromosome 12p13 . developmentofcos00belk Year: 1998 CM rS U5 A < O O CJl CM «2 -cM''-''-''=''=' Ol T- O 1â CD OOinOiCMCOVOgpCM y T j-h j i_ /> «- CMcMCMrcMCMCMCMCMOJjicM. \ C123D6 â â ,-'' \ sisS Figure 5. STS map of Chromosome 12pl3. The sixteen markers, listed along the top of the figure, are being used in the current research. Five cosmids are shown mapped to their respective markers within the lower portion of the figure. (Davies, Jeffress Grant progress report 1996). Production OF A Physical Map. In order to produce a physical map there are several steps that must occur in a specific order (FIGURE 6). First the cosmids which contains the inserted DNA from chromosome 12 must be replicated to produce a large amount for use in the next procedures. Following cosmid replication there are two possible scenarios; the cosmid DNA can be used as a template in the polymerase chain reaction (PCR) in an attempt to replicate a particular Sequence-Tagged Site marker found within the DNA sequence or it can be aliquoted onto a nylon membrane for use in hybridization procedures. If the cosmid DNA was used as a template in PCR the product is electrophoresed on an agarose gel to identify a positive or negative replication of the marker being tested. Positive replication identifies that the cosmid contains the marker sequence and places the cosmid at a physical location within the genome. If the cosmids were placed on a membrane a particular marker was then replicated via PCR and was allowed to hybridize to cosmids that contain its sequence during hybridization and detection protocols. All cosmids that 15
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