. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 1. Ouchterlony agar diffusion test. The antibody well contained ml anti-egg jelly antiserum (6612). The other wells were filled with ml of various antigen preparations. EJ: egg jelly obtained by washing eggs with acidified sea water concentrated by alcohol precipitation. CRA trypsin: cortical response antigen preparation (concentrated 100-fold) from trypsin treated eggs. CRA acid-SW: cortical response antigen preparation (concentrated 100-fold) from eggs treated with acid sea water for removal of jelly. FIGURE
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIGURE 1. Ouchterlony agar diffusion test. The antibody well contained ml anti-egg jelly antiserum (6612). The other wells were filled with ml of various antigen preparations. EJ: egg jelly obtained by washing eggs with acidified sea water concentrated by alcohol precipitation. CRA trypsin: cortical response antigen preparation (concentrated 100-fold) from trypsin treated eggs. CRA acid-SW: cortical response antigen preparation (concentrated 100-fold) from eggs treated with acid sea water for removal of jelly. FIGURE 2. Ouchterlony agar diffusion test. The antibody well contained ml anti-egg jelly antiserum (6612). The other wells were filled with ml of various antigen preparations. CRA trypsin: cortical response antigen preparation (concentrated 100-fold) from trypsin treated eggs. CRA acid-SW: cortical response antigen preparation (concentrated 100-fold) from eggs treated with acid sea water for removal of jelly. UNF trypsin: supernatant of unfertilized trypsin treated eggs (concentrated 100-fold). UNF acid-SW: supernatant of un- fertilized eggs (concentrated 100-fold) which had been dejellied with acidified sea water. When anti CRA (A-5) was diffused against a sample of the CRA preparation (from acid SW-dejellied eggs) which was originally injected into the rabbits, as many as 11 precipitin bands have been observed (Figs. 3 and 4). At least 10 of the 11 precipitin bands were continuous with 10 bands formed between anti-CRA (A-5) and the CRA preparations from fertilized trypsin treated eggs, as well as artificially activated eggs (Fig. 4). When the CRA preparations from acid sea water and trypsin treated eggs were diffused toward antiserum prepared against dejellied egg homogenates (6608) all the precipitin bands of the 2 antigen prepara- tions were continuous (Fig. 4). All of the conclusions drawn from observations of double diffusion plates were based on precipitin patterns whi
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
