. Contributions to embryology. Embryology. Fig. (3) Janus green B (Farbwerke Hoechst Company), diethylsafraninazodi- methylanilinchloride: ^' = ^ f ^ N (CH,), Fio. G. The specificity of the reaction is shown by the fact that only the latter, janus green B. the one originally recommended by Michaelis, will stain mitochondria, though the others differ only in the substitution of an Hi or (CH3)2 in the place of the (C2H5)2 group. The presence of the diethyl group in the safranin molecule is e^'idently the determining factor. Compounds containing two ethyl groups are more basic than those cont
. Contributions to embryology. Embryology. Fig. (3) Janus green B (Farbwerke Hoechst Company), diethylsafraninazodi- methylanilinchloride: ^' = ^ f ^ N (CH,), Fio. G. The specificity of the reaction is shown by the fact that only the latter, janus green B. the one originally recommended by Michaelis, will stain mitochondria, though the others differ only in the substitution of an Hi or (CH3)2 in the place of the (C2H5)2 group. The presence of the diethyl group in the safranin molecule is e^'idently the determining factor. Compounds containing two ethyl groups are more basic than those containing two methyl groups, and the compounds con- taining two methyl groups are, in turn, more basic than those with two hydrogen atoms alone, so that there is a decrease in basicity as we pass from the diethyl to the dimethyl and to the dihydrogen. This may well explain the difference in the behavior of these janus greens toward mitochondria. One would expect to find that janus green G, with the two methj'l groups, would color the mitochondria better than the janus green of Griibler, which possesses only the Ho group, but I have failed to detect any difference between the two. Both of them occasionallj'^ stain mitochondria, together with other cell structures, when in relatively high concentrations. Nevertheless, the difference in the actions of the janus green B with the two ethyl groups and the other two dj-es, together with the difference in basicity between them, would suggest that the dye actually combines chemically with the mitochondria and that the staining is not simply a process of selective absorption. Our evidence, however, is too scanty to permit us to arrive at any conclusion. The poor results obtained with some samples of the janus green are probably due to admixtures of the first and second Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of th
Size: 1596px × 1565px
Photo credit: © The Book Worm / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorcarn, bookpublisherwashingtondc, booksubjectembryology
