. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FORMS OF SEA URCHIN FERTILIZIN 263 Elcctrophoresis Freshly prepared multivalent fertilizin failed to migrate from the origin under the electrophoretic conditions employed. Univalent fertilizin formed by treatment with hydrogen peroxide is resolved into four bands, as determined by metachro- matic staining (at least 50 trials). When multivalent fertilizin is retained at room temperature for several hours a spontaneous breakdown of the material is noted from its electrophoretic behavior. With prolonged storage at 37° C, 1
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FORMS OF SEA URCHIN FERTILIZIN 263 Elcctrophoresis Freshly prepared multivalent fertilizin failed to migrate from the origin under the electrophoretic conditions employed. Univalent fertilizin formed by treatment with hydrogen peroxide is resolved into four bands, as determined by metachro- matic staining (at least 50 trials). When multivalent fertilizin is retained at room temperature for several hours a spontaneous breakdown of the material is noted from its electrophoretic behavior. With prolonged storage at 37° C, 150 hours, no material remains at the origin on the cellulose acetate strips and the M. ORIGIN U FIGURE 5. Cellulose acetate electrophoretic patterns of Lytcchinus fertilizins. The solvent was borate buffer, pH T/2 = Two hundred volts were applied for 25 minutes. M : Multivalent fertilizin. U: Univalent fertilizin formed with hydrogen peroxide. C: Univalent fertilizin formed by treatment with chymotrypsin for 30 minutes at 30° C. P: Univalent fertilizin formed by treatment with pronase for 30 minutes at 30° C. A : Autodegraded univalent fertilizin formed by storing multivalent fertilizin at 37° for 50 hours. material is functionally totally univalent. This univalent material formed by spon- taneous breakdown of the multivalent form produces a diffuse streak rather than discrete bands in cellulose acetate electrophoresis, indicating that it is more hetero- geneous than the univalent material formed by hydrogen peroxide. The rate of migration of the fastest moving fraction never exceeds that of the fastest moving component of the hydrogen peroxide formed univalent material. The spontaneous breakdown of the fertilizin may be due to the presence of digestive enzymes which were not removed during the washings of the eggs. Figure 5 shows the typical behavior of the different types of univalent fertilizin compared to the multivalent Please note that these images ar
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
