. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 246 L. S. QUACKENBUSH AND M. FINGERMAN - \ z o-30- \ OD^> c ' rh \ I o \ \ U - \ \ 0- r\n \ n N. n Sal. -5 -4 Spiperone LOG MOLAR FIGURE 8. The effect of spiperone on pigment concentration in intact crabs which were changed from a black background to a white background. Open bars are values for red pigment and hatched bars are values for black pigment. Control crabs concentrate their black and red pigments when this background change experiment is performed. Activity was calculated as described in Methods, negative values
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 246 L. S. QUACKENBUSH AND M. FINGERMAN - \ z o-30- \ OD^> c ' rh \ I o \ \ U - \ \ 0- r\n \ n N. n Sal. -5 -4 Spiperone LOG MOLAR FIGURE 8. The effect of spiperone on pigment concentration in intact crabs which were changed from a black background to a white background. Open bars are values for red pigment and hatched bars are values for black pigment. Control crabs concentrate their black and red pigments when this background change experiment is performed. Activity was calculated as described in Methods, negative values indicate inhibition. For this experiment activity values were based on 5 crabs given treatments compared to 5 crabs as control. Saline values are indicated by Sal, spiperone in saline at 10~5 M values by 5 and spiperone in saline at 10~4 M values by 4. All values are means ± one standard deviation (n = 30 each group, six replicates). eyestalk to produce extraordinarily large BPDH release, so an additional experiment was done. Eyestalks were bathed in saline containing spiperone then stimulated with 10 volts. This perfusate was then passed through the small Sephadex column and BPDH, RPCH, and BPCH bioassays were done on each fraction (Table I). BPDH activity was not affected by spiperone. Perfusates with spiperone had significantly more of the smallest RPCH than the control perfusates though no effect on the RPCH's of two other sizes were detected. Spiperone at 10~6 M completely blocked -20-i -10- u 0- BPC H. R PC H \ \ \^ I I I I S -11 -10 -9 -8 -7 -6 -5 LOG MOLAR Spiperone FIGURE 9. Dose response curve for the effect of spiperone in saline bathing isolated eyestalks stimulated with 5 volts on the presence of BPCH and RPCH measured in isolated legs. BPCH (open circles) and RPCH (open squares) activity was determined as described, negative values indicate inhibition. Increasing values mean increasing inhibition of BPCH or RPCH release as compared to controls of saline perfused
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
