. Electron microscopy; proceedings of the Stockholm Conference, September, 1956. Electron microscopy. 20 Fig. I. The preparation of formvar nets as supporting material for ullralhin tissue sections, (a) Microscope slide in 2% formvar solution, (b) Microscope slide transferred to vessel in which air is saturated with vapour of solvent for the formvar (ethylendichloride). The slide is left there for 20 min. (<) Damp air is blown into this vessel, {d) Formvar net floated off onto water surface. water the formvar net formed is floated off onto a water surface and transferred to specimen gr
. Electron microscopy; proceedings of the Stockholm Conference, September, 1956. Electron microscopy. 20 Fig. I. The preparation of formvar nets as supporting material for ullralhin tissue sections, (a) Microscope slide in 2% formvar solution, (b) Microscope slide transferred to vessel in which air is saturated with vapour of solvent for the formvar (ethylendichloride). The slide is left there for 20 min. (<) Damp air is blown into this vessel, {d) Formvar net floated off onto water surface. water the formvar net formed is floated off onto a water surface and transferred to specimen grids in the regular way. The upper two-thirds of the formvar net can be used. With this method, it is possible to prepare formvar nets with a large percentage of open area and with a rather uniform and useful size of the holes of the nets. These formvar nets can be examined in the electron microscope and the useful grids selected. Before being used, the nets should be stabilized by coating them with a layer of metal or carbon in a metal shadow casting unit. The gain in contrast when mounting the sections in nets is so considerable that thin sections can be examined without any objective aperture. This is of great advantage in high resolution electron micros- copy, as it means an elimination of the most frequent source of astigmatism. References 1. Sjostrand, F. S., Siieiue Tools 2, 25 (1955). 2. — Exptl. Cell Research 10, 657 (1956). Experiments on Staining Thin-Sections for Electron Microscopy I. R. Gibbons and J. R. G. Bradfield Cavendish Laboratory, Caitihridge Ihe development of suitable staining techniques for electron microscopy is important because staining not only increases the observed contrast but also provides a possible means of obtaining cytochemical information about cell components. Experimental.—The procedure for staining is simple. Cut a ribbon of sections and pick it up on a filmed electron microscope grid in the usual way. When the sections have dried dow
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