Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . scope is attached a light-colored opaque plate whichserves to reflect the light up-ward through the colored wedgeand the cylinder to the eye ofthe observer. In making a determination of the percentage of haemo-globin by this instrument the procedure is as follows: Fill eachcompartment about three-fourths full of distilled water. Punc-ture the finger-tip or lobe of the earof the subject by means of a sterileneedle or scalpel and, as soon as adrop of
Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . scope is attached a light-colored opaque plate whichserves to reflect the light up-ward through the colored wedgeand the cylinder to the eye ofthe observer. In making a determination of the percentage of haemo-globin by this instrument the procedure is as follows: Fill eachcompartment about three-fourths full of distilled water. Punc-ture the finger-tip or lobe of the earof the subject by means of a sterileneedle or scalpel and, as soon as adrop of blood appears, place one endof the capillary pipette (Fig. 65),which accompanies the instrument,against the drop and allow it to fill bycapillary attraction. To prevent theblood from adhering to the exteriorof the tube, and so render the deter-mination inaccurate, it is customary to apply a very thincoating of mutton fat to the outer surface before using or to1 The scale of the ordinary instrument is usually too high. ! S< ER. (Da Costa.) Fig. 65. Pipette of Fleischls HiEMOMETER. 176 PHYSIOLOGICAL CHEMISTRY. Fig. Colored Glass Wedge of FleischlsH^emometer. (Da Costa.) wrap the tube in a piece of oily chamois when not in use. Assoon as the tube has been accurately filled with blood it shouldbe dipped into the water of one of the compartments of thecylinder and all traces of the blood washed out with water bymeans of a small dropper which accompanies the instrument. If the blood is not well dis-tributed throughout thecompartment and does notform a homogeneous solu-tion the contents of the com-partment should be mixedthoroughly by means of themetal handle of the cap-illary measuring this has been done each compartment should be completelyfilled with distilled water and the glass cover adjusted, carebeing taken that the contents of the two compartments do notmix. Now adjust the cylinder so that the compartment con-taining the pure distille
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Keywords: ., bookcentury1900, bookdecade1900, bookpublisherphiladelphiablakis
