. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SALINITY AND GLYCINE UPTAKE 233 water of the appropriate salinity for periods up to 99 hours. At various inter- mediate intervals samples from each salinity were extracted in 80% ETOH as described above, and the remaining alcohol insoluble (incorporated) components were determined as follows: tissue was washed with alcohol, blotted and homog- enized in a micro-Waring blender with enough 80% ETOH to bring the volume to 50 ml. Three 1 ml samples of each homogenate were solubilized with 1 ml of Protosol (New England Nuclear), n
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SALINITY AND GLYCINE UPTAKE 233 water of the appropriate salinity for periods up to 99 hours. At various inter- mediate intervals samples from each salinity were extracted in 80% ETOH as described above, and the remaining alcohol insoluble (incorporated) components were determined as follows: tissue was washed with alcohol, blotted and homog- enized in a micro-Waring blender with enough 80% ETOH to bring the volume to 50 ml. Three 1 ml samples of each homogenate were solubilized with 1 ml of Protosol (New England Nuclear), neutralized with glacial acetic acid and counted by liquid scintillation. The alcohol soluble and insoluble radioactivities were corrected by use of quenching curves, and the data were expressed as per cent of 84 82 _ 80. 78- 76 4 a O oo 74 72- 70. (8) —I— 15 30 45 TIME (hours) FIGURE 1. Volume regulation as a function of time. Vertical bars indicate standard deviations (SD), where n = 10 (except where noted). the total activity. This method facilitates comparisons between salinities and compensates for individual differences in total activity. Additional samples of the homogenates were quantitatively separated into protein, nucleic acids, lipids and polysaccharides (glycogen). The basic metho- dology (outlined in Table I) used was that of Shibko, Koivistoinen, Tratnyek, Newall, and Friedman (1967) with modifications as suggested by Graff (1970). After their qualitive separation, the four tissue fractions were prepared for counting as follows: first, the dried components were put into solution with 5 ml of 10% KOH, using heat where necessary. Then 1 ml of each tissue fraction was neutralized with glacial acetic acid and counted using the Beckman LS-200B. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Mar
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
