. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 510 NAOKUNITAKEDA. V. 3s .. FIGURE 13. Incorporation of sH-uridine into the nucleus of the prothoracic .glands; scale 25 M. FIGURE 14. Migration of labelled substances from the nucleus to the cytoplasm; scale 25 n. (Gersch and Sttirzebecher, 1971), and Musca domestica (Studinger and Willig, 1975). Whether the prothoracic glands contained cholesterol was first examined. In Monema flavescens, the prothoracic glands showed a positive reaction in the part of their peripheral region (Figs. 16 and 17). As mentioned above, this are
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 510 NAOKUNITAKEDA. V. 3s .. FIGURE 13. Incorporation of sH-uridine into the nucleus of the prothoracic .glands; scale 25 M. FIGURE 14. Migration of labelled substances from the nucleus to the cytoplasm; scale 25 n. (Gersch and Sttirzebecher, 1971), and Musca domestica (Studinger and Willig, 1975). Whether the prothoracic glands contained cholesterol was first examined. In Monema flavescens, the prothoracic glands showed a positive reaction in the part of their peripheral region (Figs. 16 and 17). As mentioned above, this area coin- cided with part of the well-developed agranular-ER, which had been shown to con- tain some enzymes necessary for steroid hormone synthesis from cholesterol ( Shikita and Tamaoki, 1964; Clayton, 1969). Positive results were also found in the pro- thoracic glands of both diapausing and post-diapausing prepupae. It was more prominent in the post-diapausing stage (Fig. 17) than that of the diapausing stage (Fig. 16). The reaction was not uniform in the cytoplasm but always localized in the peripheral region of the gland cells. No detectable cholesterol was found in the muscle tissues used as controls (Fig. 15). The existence of cholesterol is highly- significant for ecdysone biosynthesis. Incorporation of *4C-cholesterol into the prothoracic glands. Since insects are incapable of synthesizing cholesterol, cholesterol found in the prothoracic glands originates in food. The incorporation of cholesterol from the hemolymph to the prothoracic glands was examined by the autoradiography of 14C-cholesterol. In the prothoracic glands of diapausing prepupae, the incorporation was not found, while it was clearly detected in post-diapausing prepupae (Fig. 18). These results were compared further with that of the secretory substance synthesis with the progress of diapause break. With the progress of secretory substance synthesis,. Please note that these images are extracted from scanned
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
